Spectroscopic imaging with spectral domain visible light optical coherence microscopy in Alzheimer's disease brain samples

被引：53

作者：

Lichtenegger, Antonia ^{[1
]}

Harper, Danielle J. ^{[1
]}

Augustin, Marco ^{[1
]}

Eugui, Pablo ^{[1
]}

Muck, Martina ^{[1
,2
,3
]}

Gesperger, Johanna ^{[2
,3
]}

Hitzenberger, Christoph K. ^{[1
]}

Woehrer, Adelheid ^{[2
,3
]}

Baumann, Bernhard ^{[1
]}

机构：

[1] Med Univ Vienna, Ctr Med Phys & Biomed Engn, Waehringer Guertel 18-20, A-1090 Vienna, Austria

[2] Gen Hosp, Inst Neurol, Waehringer Guertel 18-20, A-1090 Vienna, Austria

[3] Med Univ Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria

来源：

BIOMEDICAL OPTICS EXPRESS | 2017年 / 8卷 / 09期

基金：

欧洲研究理事会; 奥地利科学基金会;

关键词：

IN-VIVO; ULTRAHIGH-RESOLUTION; HIGH-SPEED; TOMOGRAPHY; TISSUE; PLAQUES; OCT; RECONSTRUCTION; MODEL;

D O I：

10.1364/BOE.8.004007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A visible light spectral domain optical coherence microscopy system was developed. A high axial resolution of 0 : 88 mu m in tissue was achieved using a broad visible light spectrum (425 685 nm). Healthy human brain tissue was imaged to quantify the difference between white (WM) and grey matter (GM) in intensity and attenuation. The high axial resolution enables the investigation of amyloid-beta plaques of various sizes in human brain tissue and animal models of Alzheimer's disease (AD). By performing a spectroscopic analysis of the OCM data, differences in the characteristics for WM, GM, and neuritic amyloid-beta plaques were found. To gain additional contrast, Congo red stained AD brain tissue was investigated. A first effort was made to investigate optically cleared mouse brain tissue to increase the penetration depth and visualize hyperscattering structures in deeper cortical regions. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License.

引用

页码：4007 / 4025

页数：19

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