Applications of single-cell sequencing in cancer research: progress and perspectives

被引：386

作者：

Lei, Yalan ^{[1
,2
,3
,4
]}

Tang, Rong ^{[1
,2
,3
,4
]}

Xu, Jin ^{[1
,2
,3
,4
]}

Wang, Wei ^{[1
,2
,3
,4
]}

Zhang, Bo ^{[1
,2
,3
,4
]}

Liu, Jiang ^{[1
,2
,3
,4
]}

Yu, Xianjun ^{[1
,2
,3
,4
]}

Shi, Si ^{[1
,2
,3
,4
]}

机构：

[1] Fudan Univ, Dept Pancreat Surg, Shanghai Canc Ctr, 270 DongAn Rd, Shanghai 200032, Peoples R China

[2] Fudan Univ, Shanghai Med Coll, Dept Oncol, Shanghai, Peoples R China

[3] Shanghai Pancreat Canc Inst, 270 DongAn Rd, Shanghai 200032, Peoples R China

[4] Fudan Univ, Pancreat Canc Inst, Shanghai, Peoples R China

来源：

JOURNAL OF HEMATOLOGY & ONCOLOGY | 2021年 / 14卷 / 01期

基金：

中国国家自然科学基金; 美国国家科学基金会;

关键词：

Single-cell sequencing; Heterogeneity; Microenvironment; Circulating tumor cells; PANCREATIC DUCTAL ADENOCARCINOMA; NUCLEUS RNA-SEQ; INTRA-TUMOR HETEROGENEITY; INFILTRATING T-CELLS; GENE-EXPRESSION; TRANSCRIPTOME ANALYSIS; MOLECULAR SIGNATURES; ANALYSIS REVEALS; INTRATUMORAL HETEROGENEITY; EPIGENETIC REGULATION;

D O I：

10.1186/s13045-021-01105-2

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Single-cell sequencing, including genomics, transcriptomics, epigenomics, proteomics and metabolomics sequencing, is a powerful tool to decipher the cellular and molecular landscape at a single-cell resolution, unlike bulk sequencing, which provides averaged data. The use of single-cell sequencing in cancer research has revolutionized our understanding of the biological characteristics and dynamics within cancer lesions. In this review, we summarize emerging single-cell sequencing technologies and recent cancer research progress obtained by single-cell sequencing, including information related to the landscapes of malignant cells and immune cells, tumor heterogeneity, circulating tumor cells and the underlying mechanisms of tumor biological behaviors. Overall, the prospects of single-cell sequencing in facilitating diagnosis, targeted therapy and prognostic prediction among a spectrum of tumors are bright. In the near future, advances in single-cell sequencing will undoubtedly improve our understanding of the biological characteristics of tumors and highlight potential precise therapeutic targets for patients.

引用

页数：26