Cotton miR393-TIR1 Module Regulates Plant Defense Against Verticillium dahliae via Auxin Perception and Signaling

被引：9
|
作者：
Shi, Gege [1 ,2 ]
Wang, Saisai [1 ,2 ]
Wang, Peng [2 ]
Zhan, Jingjing [2 ]
Tang, Ye [3 ]
Zhao, Ge [1 ]
Li, Fuguang [1 ,2 ]
Ge, Xiaoyang [1 ,2 ]
Wu, Jiahe [1 ,3 ]
机构：
[1] Zhengzhou Univ, Sch Agr Sci, State Key Lab Cotton Biol, Zhengzhou Res Base, Zhengzhou, Peoples R China
[2] Chinese Acad Agr Sci, State Key Lab Cotton Biol, Inst Cotton Res, Anyang, Peoples R China
[3] Chinese Acad Sci, Inst Microbiol, State Key Lab Plant Genom, Beijing, Peoples R China
来源：
FRONTIERS IN PLANT SCIENCE | 2022年 / 13卷
基金：
中国国家自然科学基金;
关键词：
Gossypium hirsutum; Verticillium dahliae; GhTIR1; auxin; salicylic acid; PSEUDOMONAS-SYRINGAE; REPRESSION; MICRORNAS; MECHANISM; TIR1; SUSCEPTIBILITY; EXPRESSION; RESISTANCE; VIRULENCE; YIELD;
D O I：
10.3389/fpls.2022.888703
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Plant auxin is essential in plant growth and development. However, the molecular mechanisms of auxin involvement in plant immunity are unclear. Here, we addressed the function of the cotton (Gossypium hirsutum) miR393-TIR1 module in plant defense against Verticillium dahliae infection via auxin perception and signaling. GhTIR1 was directedly cleaved by ghr-miR393 according to mRNA degradome data, 5 '-RACE analysis, and a GUS reporter assay. Ghr-miR393 knockdown significantly increased plant susceptibility to V. dahliae compared to the control, while ghr-miR393 overexpression and GhTIR1 knockdown significantly increased plant resistance. External indole-3-acetic acid (IAA) application significantly enhanced susceptibility to V. dahliae in ghr-miR393 knockdown and control plants compared to mock treatment, and only slightly increased susceptibility in overexpressing ghr-miR393 and GhTIR1-silenced plants. Application of external PEO-IAA (an auxin antagonist) had a contrary trend with IAA application. Based on yeast two-hybrid and bimolecular fluorescence complementation assays, GhTIR1 interacted with GhIAA14 in the nucleus, and GhIAA14 knockdown reduced plant resistance to V. dahliae infection. The results suggested that the ghr-miR393-GhTIR1 module regulates plant defense via auxin perception and signaling. Additionally, simultaneous knockdown of GhTIR1 and GhICS1 significantly increased plant susceptibility to V. dahliae compared to the control, indicating that salicylic acid (SA) accumulation is vital for the ghr-miR393-GhTIR1 module to regulates plant resistance. Transcriptome data also demonstrated that GhTIR1 knockdown significantly downregulated expression of auxin-related genes and upregulated expression of SA-related genes. Overall, the ghr-miR393-GhTIR1 module participates in plant response to V. dahliae infection via IAA perception and signaling partially depending on the SA defense pathway.
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页数：16
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