Galacto-oligosaccharides as an anti-bacterial and anti-invasive agent in lung infections

被引:10
|
作者
Cai, Yang [1 ]
van Putten, Jos P. M. S. [2 ]
Gilbert, Myrthe S. [3 ]
Gerrits, Walter J. J. [3 ]
Folkerts, Gert [1 ]
Braber, Saskia [1 ]
机构
[1] Univ Utrecht, Utrecht Inst Pharmaceut Sci, Fac Sci, Div Pharmacol, NL-3584 CG Utrecht, Netherlands
[2] Univ Utrecht, Dept Infect Dis & Immunol, NL-3584 CL Utrecht, Netherlands
[3] Wageningen Univ, Anim Nutr Grp, NL-6700 AH Wageningen, Netherlands
关键词
Carbohydrate-based biomaterials; Airway inflammation; Intranasal drug delivery; Bactericidal effect; Antibiotic adjuvant; Respiratory pathogens; MANNHEIMIA-HAEMOLYTICA; BRONCHOALVEOLAR LAVAGE; CALVES; MANIFESTATIONS; MICROBIOTA; MIXTURE; MODELS;
D O I
10.1016/j.biomaterials.2022.121461
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Emerging antimicrobial resistance in infections asks for novel intervention strategies. Galacto-oligosaccharides (GOS) might be attractive alternatives to antibiotics due to their anti-inflammatory and anti-adhesive properties. Mannheimia haemolytica is one of the major Pasteurellaceae associated with bovine lung infections. Using M. haemolytica, we demonstrated that GOS have the capacity to reduce bacterial viability and can be used as adjuvant to improve antibiotic efficacy. Using M. haemolytica-treated primary bronchial epithelial cells (PBECs) of calves, we identified the anti-adhesive and anti-invasive activities of GOS. The observed inhibition of cytokine/chemokine release and the prevention of airway epithelial barrier dysfunction in M. haemolytica-treated PBECs by GOS might be related to the downregulation of "toll-like receptor 4/nuclear factor-kappa B" pathway and the anti-invasive and anti-adhesive properties of GOS. Particularly, GOS lowered lipopolysaccharides- but not flagellin-induced cytokine/chemokine release in calf and human airway epithelial cells. Finally, we performed in vivo experiments in calves and demonstrated for the first time that intranasal application of GOS can relieve lung infections/inflammation and lower M. haemolytica positivity in the lungs without affecting clinical performance. These findings not only shed light on the anti-inflammatory mechanisms of GOS during lung infections, but GOS might also be a promising anti-bacterial agent for preventing (lung) infections.
引用
收藏
页数:18
相关论文
共 50 条
  • [1] Anti-obesity effects of galacto-oligosaccharides in obese rats
    Kong, Shang
    Huang, Xingjun
    Cao, Hua
    Bai, Yan
    Che, Qishi
    Nie, Hong
    Su, Zhengquan
    EUROPEAN JOURNAL OF PHARMACOLOGY, 2022, 917
  • [2] Galacto-Oligosaccharides as an Anti-Infective and Anti-Microbial Agent for Macrolide-Resistant and -Sensitive Mycoplasma pneumoniae
    Zhu, Hongzhen
    Cai, Yang
    Slimmen, Lisa J. M.
    de Bruijn, Adrianus C. J. M.
    van Rossum, Annemarie M. C.
    Folkerts, Gert
    Braber, Saskia
    Unger, Wendy W. J.
    PATHOGENS, 2023, 12 (05):
  • [3] ANTI-BACTERIAL THERAPY AND HOSPITAL INFECTIONS
    VOROPAEVA, SD
    ANKIRSKAYA, AS
    ABRAMOVA, ZI
    MIRONOVA, TG
    ANTIBIOTIKI, 1980, 25 (08): : 619 - 622
  • [5] Curcumin nanoparticles are a promising anti-bacterial and anti-inflammatory agent for treating periprosthetic joint infections
    Peng, Kuo-Ti
    Chiang, Yao-Chang
    Huang, Tsung-Yu
    Chen, Pei-Chun
    Chang, Pey-Jium
    Lee, Chiang-Wen
    INTERNATIONAL JOURNAL OF NANOMEDICINE, 2019, 14 : 469 - 481
  • [6] Biodegradation and Anti-bacterial Properties of PLA and Wood/PLA Composites Incorporated with Zeomic Anti-bacterial Agent
    Prapruddivongs, Chana
    Sombatsompop, Narongrit
    MULTI-FUNCTIONAL MATERIALS AND STRUCTURES IV, 2013, 747 : 111 - 114
  • [7] ASPECTS ON ANTI-BACTERIAL TREATMENT OF ANAEROBIC INFECTIONS
    NORDBRING, F
    NORD, CE
    SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES, 1982, : 59 - 62
  • [8] Thymol nanospheres as an effective anti-bacterial agent
    Wattanasatcha, Anna
    Rengpipat, Sirirat
    Wanichwecharungruang, Supason
    INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2012, 434 (1-2) : 360 - 365
  • [9] ANTI-BACTERIAL HOST DEFENSE IN THE BOVINE LUNG
    RICHARDS, AB
    RENSHAW, HW
    TEXAS AGRICULTURAL EXPERIMENT STATION PROGRESS REPORT, 1981, (3758): : 129 - 130
  • [10] Combining 2′-fucosyllactose and galacto-oligosaccharides exerts anti-inflammatory effects and promotes gut health
    Park, Sewon
    Park, Yoonhee
    Jeong, Yu-Jin
    Oh, Jun Gu
    Yoo, Hee Joon
    Yang, Jiyeon
    Kwon, Jung-il
    Lee, Kwang-Won
    JOURNAL OF DAIRY SCIENCE, 2024, 107 (12) : 10203 - 10220