Effects of ISSo2 insertions in structural and regulatory genes of the trimethylamine oxide reductase of Shewanella oneidensis

被引：29

作者：

Bordi, C ^{[1
]}

Iobbi-Nivol, C ^{[1
]}

Méjean, V ^{[1
]}

Patte, JC ^{[1
]}

机构：

[1] CNRS, Inst Biol Struct & Microbiol, Chim Bacterienne Lab, F-13402 Marseille 20, France

来源：

JOURNAL OF BACTERIOLOGY | 2003年 / 185卷 / 06期

关键词：

D O I：

10.1128/JB.185.6.2042-2045.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have isolated three Shewanella oneidensis mutants specifically impaired in trimethylamine oxide (TMAO) respiration. The mutations arose from insertions of an ISSo2 element into torA, torR, and torS, encoding, respectively, the TMAO reductase TorA, the response regulator TorR, and the sensor TorS. Although TorA is not the sole enzyme reducing TMAO in S. oneidensis, growth analysis showed that it is the main respiratory TMAO reductase. Use of a plasmid-borne torE'-lacZ fusion confirmed that the TorS-TorR phosphorelay mediates TNMO induction of the torECAD operon.

引用

页码：2042 / 2045

页数：4

共 27 条

[1] Rapid dephosphorylation of the TorR response regulator by the TorS unorthodox sensor in Escherichia coli [J].

Ansaldi, M ;

Jourlin-Castelli, C ;

Lepelletier, M ;

Théraulaz, L ;

Méjean, V .

JOURNAL OF BACTERIOLOGY, 2001, 183 (08) :2691-2695

[2] The TorR high-affinity binding site plays a key role in both torR autoregulation and torCAD operon expression in Escherichia coli [J].

Ansaldi, M ;

Simon, G ;

Lepelletier, M ;

Méjean, V .

JOURNAL OF BACTERIOLOGY, 2000, 182 (04) :961-966

[3] BACTERIAL REDUCTION OF TRIMETHYLAMINE OXIDE [J].

BARRETT, EL ;

KWAN, HS .

ANNUAL REVIEW OF MICROBIOLOGY, 1985, 39 :131-149

[4]

Beliaev AS, 1998, J BACTERIOL, V180, P6292

[5] CONSTRUCTION AND CHARACTERIZATION OF AMPLIFIABLE MULTICOPY DNA CLONING VEHICLES DERIVED FROM P15A CRYPTIC MINIPLASMID [J].

CHANG, ACY ;

COHEN, SN .

JOURNAL OF BACTERIOLOGY, 1978, 134 (03) :1141-1156

[6] Molecular analysis of the trimethylamine N-oxide (TMAO) reductase respiratory system from a Shewanella species [J].

Dos Santos, JP ;

Iobbi-Nivol, C ;

Couillault, C ;

Giordano, G ;

Méjean, V .

JOURNAL OF MOLECULAR BIOLOGY, 1998, 284 (02) :421-433

[7] ANAEROBIC CONTROL OF COLICIN-E1 PRODUCTION [J].

ERASO, JM ;

WEINSTOCK, GM .

JOURNAL OF BACTERIOLOGY, 1992, 174 (15) :5101-5109

[8] Electron transfer and binding of the c-type cytochrome TorC to the trimethylamine N-oxide reductase in Escherichia coli [J].

Gon, S ;

Giudici-Orticoni, MT ;

Méjean, V ;

Iobbi-Nivol, C .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (15) :11545-11551

[9] Reconstitution of the trimethylamine oxide reductase regulatory elements of Shewanella oneidensis in Escherichia coli [J].

Gon, S ;

Patte, JC ;

Dos Santos, JP ;

Méjean, V .

JOURNAL OF BACTERIOLOGY, 2002, 184 (05) :1262-1269

[10] An unsuspected autoregulatory pathway involving apocytochrome TorC and sensor TorS in Escherichia coli [J].

Gon, S ;

Jourlin-Castelli, C ;

Théraulaz, L ;

Méjean, V .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (20) :11615-11620

← 1 2 3 →