A novel non-invasive prenatal test technique with genosensor

Purpose: The aim of this study is to develop a new procedure with nanopolymer based genosensor for the determination of paternal mutation from cell-free fetal DNA in maternal blood. Materials and Methods: beta-thalassemia IVS1-110 mutation analysis was performed using quartz-crystal microbalance (QCM) and biosensor technologies. For this purpose, the probes of the mutation were immobilized on the genosensor electrode surface by means of Poly HemaMAC nanopolymer and then samples were added to the working cell for detection. Optimization, characterization, sensitivity and specificity studies of the genosensor were performed. Results: Biosensor measurements were obtained between -0.6 and 0.8 V in a constant current range by cyclic voltammetry. Common results were obtained with ARMS method in fetuses who had non-invasive prenatal diagnosis tests. When the ARMS results were compared with the genosensor results statistically, a 99% concordance rate was obtained in the ROC curve drawn. Conclusions: The developed biosensor can detect the presence of paternal mutation of the fetus specific to the probe on its surface. Compared with the traditional methods used in routine, it was found to be more costeffective, faster, with high specificity and detection efficiency.