Evaluation of recombinant multi-epitope proteins for diagnosis of goat schistosomiasis by enzyme-linked immunosorbent assay

被引：22

作者：

Lv, Chao ^{[1
,3
]}

Hong, Yang ^{[1
]}

Fu, Zhiqiang ^{[1
]}

Lu, Ke ^{[1
]}

Cao, Xiaodan ^{[1
]}

Wang, Tao ^{[1
]}

Zhu, Chuangang ^{[1
]}

Li, Hao ^{[1
]}

Xu, Rui ^{[1
]}

Jia, Bingguang ^{[1
]}

Han, Qian ^{[1
]}

Dou, Xuefeng ^{[1
,3
]}

Shen, Yuanxi ^{[1
,3
]}

Zhang, Zuhang ^{[1
]}

Zai, Jinli ^{[1
]}

Feng, Jintao ^{[1
]}

Lin, Jiaojiao ^{[1
,2
]}

机构：

[1] Chinese Acad Agr Sci, Minist Agr China, Shanghai Vet Res Inst, Key Lab Anim Parasitol, Shanghai, Peoples R China

[2] Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China

[3] Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R China

来源：

PARASITES & VECTORS | 2016年 / 9卷

关键词：

Recombinant multi-epitope proteins; Diagnosis; Goat schistosomiasis; ELISA; REAL-TIME PCR; REPUBLIC-OF-CHINA; JAPONICUM INFECTION; MEMBRANE-PROTEIN; WATER-BUFFALOS; ENDEMIC AREAS; SERODIAGNOSIS; MANSONI; PEPTIDE; ANTIGEN;

D O I：

10.1186/s13071-016-1418-4

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Background: Schistosomiasis is a huge threat to human and animal health. Apart from bovines, goats play an important role in the transmission of schistosomiasis in some endemic areas of China. An accessible, quality-assured goat schistosomiasis diagnostic technique is needed. Recently, our laboratory identified two recombinant diagnostic antigens, SjPGM and SjRAD23 via an immuno-proteomic method. The application of these two recombinant antigens to develop a higher sensitivity and specificity technique for the sheep schistosomiasis diagnosis is urgently needed. Methods: Epitopes of SjPGM and SjRAD23 were predicted and three polypeptides, two from SjRAD23 and one from SjPGM, were selected. Recombinant plasmids containing two to three DNA sequences encoding predicted polypeptides or large hydrophilic region of Sj23 (LHD-Sj23) were constructed and expressed. Eight recombinant schistosome antigens including four multi-epitope proteins and four recombinant single-molecule antigens as well as SEA, were assessed by ELISA in 91 sera from schistosome-infected goats, 44 sera from non-infected goats, 37 sera from Orientobilharzia-infected goats, and 12 from Haemonchus contortus-infected goats. Results: ELISA tests showed that three multi-epitope proteins had higher sensitivity than the four single-molecule antigens (rSjRAD23, rSjPGM, rBSjRAD23-1, rBSj23) and the multi-epitope protein rBSjPGM-BSjRAD23-1-BSj23 had the highest sensitivity (97.8 %, 89/91) and maintained good specificity (100 %, 44/44) as well as low cross-reactivity with haemonchosis (8.33 %, 3/12) and orientobilharziasis (13.51 %, 5/37) in the diagnosis of goat schistosomiasis. In contrast, when SEA was applied as a diagnosis antigen, it had 100 % (91/91) sensitivity, 75 % (33/44) specificity, 25 and 83.78 % cross-reactivity with haemonchosis (3/12) and orientobilharziasis (31/37), respectively. Conclusions: The application of recombinant multi-epitope proteins may increase the sensitivity of diagnosis technique and retain high specificity of single-molecule antigens for schistosomiasis, and the recombinant antigen rBSjPGM-BSjRAD23-1-BSj23 has the potential to be used as a diagnosis antigen for goat schistosomiasis.

引用

页数：11

共 40 条

[1] Utilization of ELISA Using Thioredoxin Peroxidase-1 and Tandem Repeat Proteins for Diagnosis of Schistosoma japonicum Infection among Water Buffaloes [J].