Efficient, dual-stimuli responsive cytosolic gene delivery using a RGD modified disulfide-linked polyethylenimine functionalized gold nanorod

被引:57
作者
Wang, Feihu [1 ]
Shen, Yuanyuan [1 ]
Zhang, Wenjun [1 ]
Li, Min [1 ]
Wang, Yun [1 ]
Zhou, Dejian [2 ]
Guo, Shengrong [1 ,2 ]
机构
[1] Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China
[2] Univ Leeds, Sch Chem, Astbury Ctr Struct Mol Biol, Leeds LS2 9JT, W Yorkshire, England
基金
欧洲研究理事会; 英国工程与自然科学研究理事会; 中国国家自然科学基金;
关键词
Gold nanorod; Disulfide-linked polyethylenimine; Glutathione; Near-infrared laser; Photochemical effect; Controlled gene delivery; LOW-MOLECULAR-WEIGHT; DRUG-DELIVERY; CANCER-CELLS; CLICK CHEMISTRY; TRIGGERED DRUG; IN-VITRO; RELEASE; NANOPARTICLES; DNA; PROGRESS;
D O I
10.1016/j.jconrel.2014.09.026
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Controlled-release systems capable of responding to external stimuli and/or unique internal environments have received great interests in site-specific gene and/or drug delivery. In this work, a functionalized gene nanocarrier for dual-stimuli triggered cytosolic gene delivery is developed and showing high gene delivery efficacy with low cytotoxicity. The nanocarrier is prepared by conjugating gold nanorod (GNR) with multiple disulfide cross-linked short PEIs to harness the advantageous properties of GNR based near infrared (NIR) laser induced photothermal heating and intracellular stimuli-triggered degradability of disulfide cross-linked short PEIs (DSPEI). The DSPEI is further grafted with a poly(ethylene glycol) (PEG) section to afford high carrier stability in cell cultures and a terminal RGD peptide for specific targeting of cancer cells. The nanocarrier is found to effectively condense plasmid DNA to form a highly stable GNR-DSPEI-PEG-RGD/DNA complex with tumor cell-targeting ability that can be efficiently uptaken by cancer cells. Moreover, the loaded genes can be effectively released from the complex triggered by the high intracellular glutathione content and/or by photothermal effect of NIR irradiation at 808 nm. Interestingly, the GNRs-based complex can easily escape from intracellular endo-/lyso-somal compartments and release the gene load into the cytosol upon exposure to NIR irradiation, resulting in significantly improved gene transfection efficiency. Our new gene carrier exhibits high gene transfection efficiency, comparable to or even better than that of high MW PEIs, but with a much lower cytotoxicity. Additionally, neither the GNR-based carrier nor the laser treatment shows any significant evidence of cytotoxicity. This work demonstrates a promising strategy for intracellular stimuli triggered, photothermal controllable gene delivery system, which can be further applied to many other nanomedicine fields. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:37 / 51
页数:15
相关论文
共 74 条
[41]   Optical properties and ultrafast dynamics of metallic nanocrystals [J].
Link, S ;
Ei-Sayed, MA .
ANNUAL REVIEW OF PHYSICAL CHEMISTRY, 2003, 54 :331-366
[42]   Novel Reduction-Responsive Cross-Linked Polyethylenimine Derivatives by Click Chemistry for Nonviral Gene Delivery [J].
Liu, Jia ;
Jiang, Xulin ;
Xu, Li ;
Wang, Xianmiao ;
Hennink, Wim E. ;
Zhuo, Renxi .
BIOCONJUGATE CHEMISTRY, 2010, 21 (10) :1827-1835
[43]   Biomedical nanoparticle carriers with combined thermal and magnetic responses [J].
Liu, Ting-Yu ;
Hu, Shang-Hsiu ;
Liu, Dean-Mo ;
Chen, San-Yuan ;
Chen, I-Wei .
NANO TODAY, 2009, 4 (01) :52-65
[44]   Plasmonic nanobubble-enhanced endosomal escape processes for selective and guided intracellular delivery of chemotherapy to drug-resistant cancer cells [J].
Lukianova-Hleb, Ekaterina Y. ;
Belyanin, Andrey ;
Kashinath, Shruti ;
Wu, Xiangwei ;
Lapotko, Dmitri O. .
BIOMATERIALS, 2012, 33 (06) :1821-1826
[45]   Anisotropic metal nanoparticles: Synthesis, assembly, and optical applications [J].
Murphy, CJ ;
San, TK ;
Gole, AM ;
Orendorff, CJ ;
Gao, JX ;
Gou, L ;
Hunyadi, SE ;
Li, T .
JOURNAL OF PHYSICAL CHEMISTRY B, 2005, 109 (29) :13857-13870
[46]   Stabilized nanocarriers for plasmids based upon cross-linked poly(ethylene imine) [J].
Neu, Michael ;
Sitterberg, Johannes ;
Bakowsky, Udo ;
Kissel, Thomas .
BIOMACROMOLECULES, 2006, 7 (12) :3428-3438
[47]   Poly(ethylene glycol)-Modified Gold Nanorods as a Photothermal Nanodevice for Hyperthermia [J].
Niidome, Takuro ;
Akiyama, Yasuyuki ;
Yamagata, Masato ;
Kawano, Takahito ;
Mori, Takeshi ;
Niidome, Yasuro ;
Katayama, Yoshiki .
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 2009, 20 (09) :1203-1215
[48]  
Nikoobakht B, 2003, CHEM MATER, V15, P1957, DOI [10.1021/cm020732l, 10.1021/cm0207321]
[49]   Light-Addressable Capsules as Caged Compound Matrix for Controlled Triggering of Cytosolic Reactions [J].
Ochs, Markus ;
Carregal-Romero, Susana ;
Rejman, Joanna ;
Braeckmans, Kevin ;
De Smedt, Stefaan C. ;
Parak, Wolfgang J. .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2013, 52 (02) :695-699
[50]   Controlled self-assembly of thiol-terminated poly(amidoamine) dendrimer and gold nanoparticles [J].
Pan, BF ;
Gao, F ;
Ao, LM ;
Tian, HY ;
He, R ;
Cui, DX .
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2005, 259 (1-3) :89-94