Controlled-release systems capable of responding to external stimuli and/or unique internal environments have received great interests in site-specific gene and/or drug delivery. In this work, a functionalized gene nanocarrier for dual-stimuli triggered cytosolic gene delivery is developed and showing high gene delivery efficacy with low cytotoxicity. The nanocarrier is prepared by conjugating gold nanorod (GNR) with multiple disulfide cross-linked short PEIs to harness the advantageous properties of GNR based near infrared (NIR) laser induced photothermal heating and intracellular stimuli-triggered degradability of disulfide cross-linked short PEIs (DSPEI). The DSPEI is further grafted with a poly(ethylene glycol) (PEG) section to afford high carrier stability in cell cultures and a terminal RGD peptide for specific targeting of cancer cells. The nanocarrier is found to effectively condense plasmid DNA to form a highly stable GNR-DSPEI-PEG-RGD/DNA complex with tumor cell-targeting ability that can be efficiently uptaken by cancer cells. Moreover, the loaded genes can be effectively released from the complex triggered by the high intracellular glutathione content and/or by photothermal effect of NIR irradiation at 808 nm. Interestingly, the GNRs-based complex can easily escape from intracellular endo-/lyso-somal compartments and release the gene load into the cytosol upon exposure to NIR irradiation, resulting in significantly improved gene transfection efficiency. Our new gene carrier exhibits high gene transfection efficiency, comparable to or even better than that of high MW PEIs, but with a much lower cytotoxicity. Additionally, neither the GNR-based carrier nor the laser treatment shows any significant evidence of cytotoxicity. This work demonstrates a promising strategy for intracellular stimuli triggered, photothermal controllable gene delivery system, which can be further applied to many other nanomedicine fields. (C) 2014 Elsevier B.V. All rights reserved.
机构:
Univ Penn, Dept Mat Sci & Engn, Philadelphia, PA 19104 USA
Natl Chiao Tung Univ, Dept Mat Sci & Engn, Hsinchu, TaiwanUniv Penn, Dept Mat Sci & Engn, Philadelphia, PA 19104 USA
Liu, Ting-Yu
;
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Hu, Shang-Hsiu
;
Liu, Dean-Mo
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Natl Chiao Tung Univ, Dept Mat Sci & Engn, Hsinchu, TaiwanUniv Penn, Dept Mat Sci & Engn, Philadelphia, PA 19104 USA
Liu, Dean-Mo
;
Chen, San-Yuan
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Natl Chiao Tung Univ, Dept Mat Sci & Engn, Hsinchu, TaiwanUniv Penn, Dept Mat Sci & Engn, Philadelphia, PA 19104 USA
Chen, San-Yuan
;
Chen, I-Wei
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Univ Penn, Dept Mat Sci & Engn, Philadelphia, PA 19104 USAUniv Penn, Dept Mat Sci & Engn, Philadelphia, PA 19104 USA
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Kyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
Kyushu Univ, Ctr Future Chem, Nishi Ku, Fukuoka 8190395, Japan
Japan Sci & Technol Corp, PRESTO, Kawaguchi, Saitama 3320012, JapanKyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
Niidome, Takuro
;
Akiyama, Yasuyuki
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Kyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, JapanKyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
Akiyama, Yasuyuki
;
Yamagata, Masato
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Kyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, JapanKyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
Yamagata, Masato
;
Kawano, Takahito
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Kyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, JapanKyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
Kawano, Takahito
;
Mori, Takeshi
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Kyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
Kyushu Univ, Ctr Future Chem, Nishi Ku, Fukuoka 8190395, JapanKyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
Mori, Takeshi
;
Niidome, Yasuro
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Kyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, JapanKyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
Niidome, Yasuro
;
Katayama, Yoshiki
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Kyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
Kyushu Univ, Ctr Future Chem, Nishi Ku, Fukuoka 8190395, Japan
Japan Sci & Technol Corp, CREST, Kawaguchi, Saitama 3320012, JapanKyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
机构:
Univ Penn, Dept Mat Sci & Engn, Philadelphia, PA 19104 USA
Natl Chiao Tung Univ, Dept Mat Sci & Engn, Hsinchu, TaiwanUniv Penn, Dept Mat Sci & Engn, Philadelphia, PA 19104 USA
Liu, Ting-Yu
;
论文数: 引用数:
h-index:
机构:
Hu, Shang-Hsiu
;
Liu, Dean-Mo
论文数: 0引用数: 0
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Natl Chiao Tung Univ, Dept Mat Sci & Engn, Hsinchu, TaiwanUniv Penn, Dept Mat Sci & Engn, Philadelphia, PA 19104 USA
Liu, Dean-Mo
;
Chen, San-Yuan
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机构:
Natl Chiao Tung Univ, Dept Mat Sci & Engn, Hsinchu, TaiwanUniv Penn, Dept Mat Sci & Engn, Philadelphia, PA 19104 USA
Chen, San-Yuan
;
Chen, I-Wei
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Univ Penn, Dept Mat Sci & Engn, Philadelphia, PA 19104 USAUniv Penn, Dept Mat Sci & Engn, Philadelphia, PA 19104 USA
机构:
Kyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
Kyushu Univ, Ctr Future Chem, Nishi Ku, Fukuoka 8190395, Japan
Japan Sci & Technol Corp, PRESTO, Kawaguchi, Saitama 3320012, JapanKyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
Niidome, Takuro
;
Akiyama, Yasuyuki
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机构:
Kyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, JapanKyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
Akiyama, Yasuyuki
;
Yamagata, Masato
论文数: 0引用数: 0
h-index: 0
机构:
Kyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, JapanKyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
Yamagata, Masato
;
Kawano, Takahito
论文数: 0引用数: 0
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机构:
Kyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, JapanKyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
Kawano, Takahito
;
Mori, Takeshi
论文数: 0引用数: 0
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机构:
Kyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
Kyushu Univ, Ctr Future Chem, Nishi Ku, Fukuoka 8190395, JapanKyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
Mori, Takeshi
;
Niidome, Yasuro
论文数: 0引用数: 0
h-index: 0
机构:
Kyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, JapanKyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
Niidome, Yasuro
;
Katayama, Yoshiki
论文数: 0引用数: 0
h-index: 0
机构:
Kyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
Kyushu Univ, Ctr Future Chem, Nishi Ku, Fukuoka 8190395, Japan
Japan Sci & Technol Corp, CREST, Kawaguchi, Saitama 3320012, JapanKyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan