Comparison of real-time polymerase chain reaction and conventional biochemical methods for identification of Mycobacterium chelonae-Mycobacterium abscessus group to the species level

被引：5

作者：

Guarin, Nora ^{[3
]}

Budvytiene, Indre ^{[3
]}

Ghafghaichi, Laleh ^{[3
]}

Banaei, Niaz ^{[1
,2
,3
]}

机构：

[1] Stanford Univ, Sch Med, Dept Pathol, Stanford, CA 94305 USA

[2] Stanford Univ, Sch Med, Dept Med Infect Dis, Stanford, CA 94305 USA

[3] Stanford Hosp & Clin, Clin Microbiol Lab, Palo Alto, CA 94304 USA

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2010年 / 67卷 / 04期

关键词：

M. chelonae-M. abscessus group; M; immunogenum; Real-time PCR; Identification; RAPID IDENTIFICATION; NONTUBERCULOUS MYCOBACTERIA; PSEUDO-OUTBREAKS; PCR; DIFFERENTIATION; EXPERIENCE; GENE;

D O I：

10.1016/j.diagmicrobio.2010.03.011

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The Mycobacterium chelonae Mycobacterium abscessus group (MCAG) is the most common cause of infections because of rapidly growing mycobacteria. Rapid identification of MCAG to the species level is essential for choosing empiric antibiotic treatment and for public health measures. In this study, we compared the performance of a single-tube multiplex, real-time polymerase chain reaction (PCR) assay to 3 biochemical tests for species-level identification of 46 MCAG isolates. We show that real-time PCR provides the most accurate results for rapid species-level identification of MCAG. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：333 / 336

页数：4

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