Immunoelectron microscopic localization and analysis of Herpes simplex virus type 1 antigens

被引：0

作者：

Chung, CC ^{[1
]}

Lee, HH ^{[1
]}

Cho, MH ^{[1
]}

机构：

[1] Konkuk Univ, Dept Biol, Seoul 143701, South Korea

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2000年 / 10卷 / 05期

关键词：

Herpes simplex virus type 1; immunodominant antigen; localization; immunogold labeling; immunofluorescence;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Antigens of Herpes simplex virus type 1 (HSV-1) strain F were immunoblotted to identify the most immunodominant one, and the localization of this antigen was then studied using immunoelectron microscopy. The 67.8 kDa antigen appeared to be the most immunodominant one in a mouse model. and it showed randomly scattered and partially clustered distribution on the surface of the virion. The localization study was performed using immunogold with polyclonal anti-HSV-1 sera produced from BALB/c mice, and immunofluorescence demonstrated that the viral products in the HSV-1 infected Vero cells were distributed throughout the infected host cell, however, mainly on the surface of the host membrane.

引用

页码：714 / 720

页数：7

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