Assessment and Optimizations of Candida albicans In Vitro Biofilm Assays

被引：40

作者：

Lohse, Matthew B. ^{[1
,2
]}

Gulati, Megha ^{[3
]}

Arevalo, Ashley Valle ^{[3
,4
]}

Fishburn, Adam ^{[3
]}

Johnson, Alexander D. ^{[1
,5
]}

Nobile, Clarissa J. ^{[3
]}

机构：

[1] Univ Calif San Francisco, Dept Microbiol & Immunol, San Francisco, CA 94143 USA

[2] BioSynesis Inc, Dept Biol, San Francisco, CA USA

[3] Univ Calif, Sch Nat Sci, Dept Mol & Cell Biol, Merced, CA 95340 USA

[4] Univ Calif, Quantitat & Syst Biol Grad Program, Merced, CA USA

[5] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA USA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2017年 / 61卷 / 05期

基金：

美国国家卫生研究院;

关键词：

Candida albicans; in vitro biofilm assays; biofilm methods; biofilm protocols; biofilm screens; biofilm; ANTIFUNGAL SUSCEPTIBILITY; MODEL; QUANTIFICATION; PARAPSILOSIS; VALIDATION; VIRULENCE; SURFACE; AGENTS; GENES;

D O I：

10.1128/AAC.02749-16

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Candida albicans biofilms have a significant medical impact due to their rapid growth on implanted medical devices, their resistance to antifungal drugs, and their ability to seed disseminated infections. Biofilm assays performed in vitro allow for rapid, high-throughput screening of gene deletion libraries or antifungal compounds and typically serve as precursors to in vivo studies. Here, we compile and discuss the protocols for several recently published C. albicans in vitro biofilm assays. We also describe improved versions of these protocols as well as novel in vitro assays. Finally, we consider some of the advantages and disadvantages of these different types of assays.

引用

页数：13

共 55 条

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