Transketolase catalysed upgrading of L-arabinose: the one-step stereoselective synthesis of L-gluco-heptulose

被引：33

作者：

Subrizi, Fabiana ^{[1
]}

Cardenas-Fernandez, Max ^{[2
]}

Lye, Gary J. ^{[2
]}

Ward, John M. ^{[2
]}

Dalby, Paul A. ^{[2
]}

Sheppard, Tom D. ^{[1
]}

Hailes, Helen C. ^{[1
]}

机构：

[1] UCL, Dept Chem, 20 Gordon St, London WC1H 0AJ, England

[2] UCL, Dept Biochem Engn, Adv Ctr Biochem Engn, Bernard Katz Bldg, London WC1H 0AH, England

来源：

GREEN CHEMISTRY | 2016年 / 18卷 / 10期

基金：

英国工程与自然科学研究理事会;

关键词：

SUGAR-BEET PULP; ENZYMATIC THIAMIN CATALYSIS; D-XYLULOSE; 5-PHOSPHATE; SACCHAROMYCES-CEREVISIAE; SUBSTRATE-SPECIFICITY; DEPENDENT ENZYMES; D-MANNOHEPTULOSE; DIRECTED EVOLUTION; ALIPHATIC ALDEHYDE; MASS SPECTROMETRY;

D O I：

10.1039/c5gc02660a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Conversion of biomass using biocatalysis is likely to become a technology that contributes significantly to the future production of chemical building blocks, materials and transport fuels. Here the synthesis of a value-added chemical from L-arabinose, a major component of the carbohydrates in sugar beet pulp (SBP), in a concise and sustainable manner has been investigated. Biocatalytic conversions using transketolase variants have been developed for the efficient, scalable synthesis of a rare naturally occurring ketoheptose, L-gluco-heptulose, from L-arabinose. New active E. coli TK mutants that readily accept L-arabinose were identified using a versatile colorimetric screening assay and the reaction was performed on a preparative scale.

引用

页码：3158 / 3165

页数：8