Enhancement of the antibiotic activity of aminoglycosides by extracts from Anadenanthera colubrine (Vell.) Brenan var. cebil against multi-drug resistant bacteria

被引：14

作者：

Barreto, Humberto M. ^{[1
]}

Coelho, Kivia M. R. N. ^{[1
]}

Ferreira, Josie H. L. ^{[1
]}

dos Santos, Bernadete H. C. ^{[2
]}

de Abreu, Aislan P. L. ^{[3
]}

Coutinho, Henrique D. M. ^{[4
]}

da Silva, Romezio A. C. ^{[5
]}

de Sousa, Taciana O. ^{[5
]}

Cito, Antonia M. das G. L. ^{[5
]}

Lopes, Jose A. D. ^{[5
]}

机构：

[1] Univ Fed Piaui, Lab Res Microbiol, Teresina, PI, Brazil

[2] Univ Fed Paraiba, Lab Clin Microbiol, Joao Pessoa, PB, Brazil

[3] Fac Higher Educ Floriano, Microbiol Lab, Floriano, PI, Brazil

[4] Reg Univ Cariri, Dept Biol Chem, Lab Microbiol & Mol Biol, Crato, CE, Brazil

[5] Univ Fed Piaui, Lab Nat Prod, Teresina, PI, Brazil

来源：

NATURAL PRODUCT RESEARCH | 2016年 / 30卷 / 11期

关键词：

Anadenanthera colubrina var; cebil; Staphylococcus aureus; Escherichia coli; antimicrobial activity; modifying-resistance activity; efflux pump; chlorpromazine; STAPHYLOCOCCUS-AUREUS; ANTIMICROBIAL RESISTANCE;

D O I：

10.1080/14786419.2015.1049177

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The aim of this work was to evaluate the antimicrobial activity of ethanol (EEAC) and hexane (HFAC) extracts from the stem bark of Anadenanthera colubrina (Vell.) Brenan var. cebil alone or in combination with aminoglycosides against multi-drug resistant (MDR) bacteria. Minimal inhibitory concentrations (MICs) of the extracts were determined by using microdilution assay. For the evaluation of extracts as modulators of antibiotic resistance, MICs of neomycin and amikacin were determined in presence or absence of each compound at sub-inhibitory concentrations. Both EEAC and HFAC did not show antimicrobial activity against MDR strains tested. However, the addition of EEAC and HFAC enhanced the activity of neomycin and amikacin against Staphylococcus aureus SA10 strain. When the natural products were replaced by chlorpromazine, the same effect was observed. Anadenanthera colubrine var. cebil may be a source of phytochemicals able to potentiate the aminoglycoside activity against MDR S. aureus by the inhibition of efflux pump.

引用

页码：1289 / 1292

页数：4

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