Effect of Polysaccharide Sources on the Physicochemical Properties of Bromelain-Chitosan Nanoparticles

被引：24

作者：

Ataide, Janaina Artem ^{[1
,2
]}

Gerios, Eloah Favero ^{[2
,3
]}

Cefali, Leticia Caramori ^{[2
,4
]}

Fernandes, Ana Rita ^{[2
]}

Teixeira, Maria do Ceu ^{[2
]}

Ferreira, Nuno R. ^{[2
]}

Tambourgi, Elias Basile ^{[5
]}

Jozala, Angela Faustino ^{[6
]}

Chaud, Marco Vinicius ^{[7
]}

Oliveira-Nascimento, Laura ^{[3
]}

Mazzola, Priscila Gava ^{[3
]}

Souto, Eliana B. ^{[2
,8
]}

机构：

[1] Univ Campinas UNICAMP, Sch Med Sci, Grad Program Med Sci, BR-13010 Campinas, SP, Brazil

[2] Univ Coimbra FFUC, Fac Pharm, Dept Pharmaceut Technol, Polo Ciencias Saude, P-3000 Coimbra, Portugal

[3] Univ Campinas UNICAMP, Fac Pharmaceut Sci, BR-13010 Campinas, SP, Brazil

[4] Univ Campinas UNICAMP, Inst Biol, Grad Program Biosci & Technol Bioact Prod, BR-13010 Campinas, SP, Brazil

[5] Univ Campinas UNICAMP, Sch Chem Engn, BR-13010 Campinas, SP, Brazil

[6] Univ Sorocaba UNISO, Lab Ind Microbiol & Fermentat Proc, BR-18010 Sorocaba, Brazil

[7] Univ Sorocaba UNISO, Lab Biomat & Nanotechnol, BR-18010 Sorocaba, Brazil

[8] Univ Minho, CEB Ctr Biol Engn, Campus Gualtar, P-4700 Braga, Portugal

来源：

POLYMERS | 2019年 / 11卷 / 10期

基金：

瑞典研究理事会; 巴西圣保罗研究基金会;

关键词：

bromelain; chitosan nanoparticles; physicochemical characterization; low molecular weight chitosan; chitosan oligosaccharide lactate; chitosan from shrimp shells; PINEAPPLE ANANAS-COMOSUS; DRUG-DELIVERY; MICROSPHERES; PURIFICATION; STABILITY; DESIGN; FORMULATION; CARRIERS; RELEASE; PELLETS;

D O I：

10.3390/polym11101681

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Bromelain, a set of proteolytic enzymes potential pharmaceutical applications, was encapsulated in chitosan nanoparticles to enhance enzyme stability, and the effect of different chitosan sources was evaluated. Chitosan types (i.e., low molecular weight chitosan, chitosan oligosaccharide lactate, and chitosan from shrimp shells) produced nanoparticles with different physicochemical properties, however in all cases, particle size and zeta potential decreased, and polydispersity index increased after bromelain addition. Bromelain encapsulation was higher than 84% and 79% for protein content and enzymatic activity, respectively, with low molecular weight chitosan presenting the highest encapsulation efficiency. Nanoparticle suspension was also tested for accelerated stability and rheological behavior. For the chitosan-bromelain nanoparticles, an instability index below 0.3 was recorded and, in general, the loading of bromelain in chitosan nanoparticles decreased the cohesiveness of the final suspension.

引用

页数：13

共 56 条

[51] Bromelain-decorated hybrid nanoparticles based on lactobionic acid-conjugated chitosan for in vitro anti-tumor study [J].