DETECTION OF SEVEN NITROIMIDAZOLES AND TYLOSIN A RESIDUES IN HONEY BY LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY (LC/MS)

A simple extraction and rapid routine method for the simultaneous determination of nitroimidazoles (DMZ, MNZ, RNZ, IPZ), nitroimidazole metabolites (HMMNI, MNZOH, IPZOH), and tylosin A (TYL) in honey using liquid chromatography-tandem mass spectrometry (LC/MS) was developed and fully validated in this study. The study optimized the sample pre-treatment, high performance liquid chromatography (HPLC) mobile phase conditions, and relevant parameters of mass spectroscopy. Data was acquired by applying multiple reaction monitoring, and quantitation was performed using four internal standards. Using the criteria specified in the European Commission Regulation (EU) 37/2010 for qualitative methods, the linear calibrations were established for all target compounds and the R2 values ranged between 0.9994 and 0.9999. The recoveries ranged from 86.4% to 96.3%. The decision limits (CC alpha) and the detection capabilities (CC beta) of all target compounds were 0.0649 mu g/kg-0.1493 mu/kg and 0.1152 mu g/kg-0.2238 mu g/kg, respectively. Sensitivity, accuracy, and precision complied with the requirements of veterinary drug residue testing methods, thereby achieving an accurate and simple method.