Sargassum serratifolium Extract Attenuates Interleukin-1β-Induced Oxidative Stress and Inflammatory Response in Chondrocytes by Suppressing the Activation of NF-κB, p38 MAPK, and PI3K/Akt

被引:51
作者
Park, Cheol [1 ]
Jeong, Jin-Woo [2 ]
Lee, Dae-Sung [3 ]
Yim, Mi-Jin [3 ]
Lee, Jeong Min [3 ]
Han, Min Ho [3 ]
Kim, Suhkmann [4 ,5 ]
Kim, Heui-Soo [6 ]
Kim, Gi-Young [7 ]
Park, Eui Kyun [8 ]
Jeon, You-Jin [7 ]
Cha, Hee-Jae [9 ]
Choi, Yung Hyun [10 ,11 ,12 ]
机构
[1] Dong Eui Univ, Dept Mol Biol, Coll Nat Sci, Busan 47340, South Korea
[2] Nakdonggang Natl Inst Biol Resources, Freshwater Bioresources Utilizat Bur, Sangju 37242, South Korea
[3] Natl Marine Biodivers Inst Korea, Seocheon 33662, South Korea
[4] Pusan Natl Univ, Dept Chem, Coll Nat Sci, Ctr Proteome Biophys, Busan 46241, South Korea
[5] Pusan Natl Univ, Chem Inst Funct Mat, Busan 46241, South Korea
[6] Pusan Natl Univ, Dept Biol Sci, Coll Nat Sci, Busan 46241, South Korea
[7] Jeju Natl Univ, Sch Marine Biomed Sci, Dept Marine Life Sci, Jeju 63243, South Korea
[8] Kyungpook Natl Univ, Inst Hard Tissue & Biotooth Regenerat, Sch Dent, Dept Oral Pathol & Regenerat Med, Daegu 41940, South Korea
[9] Kosin Univ, Dept Parasitol & Genet, Coll Med, Busan 49267, South Korea
[10] Dong Eui Univ, Antiaging Res Ctr, Busan 47227, South Korea
[11] Dong Eui Univ, Blue Bio Ind RIC, Busan 47227, South Korea
[12] Dong Eui Univ, Coll Korean Med, Dept Biochem, Busan 47227, South Korea
关键词
Sargassum serratifolium; chondrocytes; inflammation; ROS; NF-kappa B; MAPKs; PI3K; Akt; NONSTEROIDAL ANTIINFLAMMATORY DRUGS; MATRIX METALLOPROTEINASES; OSTEOARTHRITIS; EXPRESSION; MEDIATORS; KNEE; CARTILAGE;
D O I
10.3390/ijms19082308
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Osteoarthritis (OA) is a degenerative joint disease that is characterized by irreversible articular cartilage destruction by inflammatory reaction. Among inflammatory stimuli, interleukin-1 (IL-1) is known to play a crucial role in OA pathogenesis by stimulating several mediators that contribute to cartilage degradation. Recently, the marine brown alga Sargassum serratifolium has been reported to exhibit antioxidant and anti-inflammatory effects in microglial and human umbilical vein endothelial cell models using lipopolysaccharide and tumor necrosis factor-, but its beneficial effects on OA have not been investigated. This study aimed to evaluate the anti-osteoarthritic effects of ethanol extract of S. serratifolium (EESS) in SW1353 human chondrocytes and, in parallel, primary rat articular chondrocytes. Our results showed that EESS effectively blocked the generation of reactive oxygen species in IL-1-treated SW1353 and rat primary chondrocytes, indicating that EESS has a potent antioxidant activity. EESS also attenuated IL-1-induced production of nitric oxide (NO) and prostaglandin E-2, major inflammatory mediators in these cells, which was associated with the inhibition of inducible NO synthase and cyclooxygenase-2 expression. Moreover, EESS downregulated the level of gene expression of matrix metalloproteinase (MMP)-1, -3 and -13 in SW1353 chondrocytes treated with IL-1, resulting in their extracellular secretion reduction. In addition, the IL-1-induced activation of nuclear factor-kappa B (NF-B) was restored by EESS. Furthermore, EESS reduced the activation of p38 mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathways upon IL-1 stimulation. These results indicate that EESS has the potential to exhibit antioxidant and anti-inflammatory effects through inactivation of the NF-B, p38 MAPK, and PI3K/Akt signaling pathways. Collectively, these findings demonstrate that EESS may have the potential for chondroprotection, and extracts of S. serratifolium could potentially be used in the prevention and treatment of OA.
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页数:15
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