Sequence of the Bacillus stearothermophilus gene encoding aspartokinase II

被引：1

作者：

Cantoni, R ^{[1
]}

Labo, M ^{[1
]}

DeRossi, E ^{[1
]}

Riccardi, G ^{[1
]}

机构：

[1] UNIV PAVIA,DIPARTIMENTO GENET & MICROBIOL,I-27100 PAVIA,ITALY

来源：

GENE | 1996年 / 169卷 / 01期

关键词：

recombinant DNA; DNA sequencing; inverse PCR;

D O I：

10.1016/0378-1119(95)00834-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The complete nucleotide sequence of the gene encoding aspartokinase (Ask) II from thermophilic Bacillus stearothermophilus has been determined. Degenerate oligodeoxyribonucleotides primed the amplification of a 932-bp gene. This sequence was successively used for constructing new primers applied in inverse polymerase chain reaction using, as template, self-ligating DNA fragments. The deduced amino-acid sequence is 68.7% identical with the sequence of the Bacillus sp. strain MGA.3 Ask II.

引用

页码：135 / 136

页数：2

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