Sequence of the Bacillus stearothermophilus gene encoding aspartokinase II

被引：1
作者：
Cantoni, R [1 ]
Labo, M [1 ]
DeRossi, E [1 ]
Riccardi, G [1 ]
机构：
[1] UNIV PAVIA,DIPARTIMENTO GENET & MICROBIOL,I-27100 PAVIA,ITALY
来源：
GENE | 1996年 / 169卷 / 01期
关键词：
recombinant DNA; DNA sequencing; inverse PCR;
D O I：
10.1016/0378-1119(95)00834-9
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The complete nucleotide sequence of the gene encoding aspartokinase (Ask) II from thermophilic Bacillus stearothermophilus has been determined. Degenerate oligodeoxyribonucleotides primed the amplification of a 932-bp gene. This sequence was successively used for constructing new primers applied in inverse polymerase chain reaction using, as template, self-ligating DNA fragments. The deduced amino-acid sequence is 68.7% identical with the sequence of the Bacillus sp. strain MGA.3 Ask II.
引用
收藏
页码：135 / 136
页数：2
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