Sox2 is involved in paclitaxel resistance of the prostate cancer cell line PC-3 via the PI3K/Akt pathway

被引：52

作者：

Li, Dong ^{[1
]}

Zhao, Li-Nan ^{[1
]}

Zheng, Xiu-Lan ^{[2
]}

Lin, Ping ^{[1
]}

Lin, Feng ^{[1
]}

Li, Yue ^{[1
]}

Zou, Hai-Feng ^{[1
]}

Cui, Rong-Jun ^{[1
]}

Chen, Hui ^{[3
]}

Yu, Xiao-Guang ^{[1
]}

机构：

[1] Harbin Med Univ, Dept Biochem & Mol Biol, Harbin 150081, Heilongjiang, Peoples R China

[2] Harbin Med Univ, Dept Ultrasonog, Canc Hosp, Harbin 150081, Heilongjiang, Peoples R China

[3] Harbin Med Univ, Dept Urol, Affiliated Hosp 2, Harbin 150081, Heilongjiang, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2014年 / 10卷 / 06期

关键词：

Sox2; paclitaxel; drug resistance; prostate cancer; phosphoinositide 3-kinase/Akt pathway; LUNG; CHEMORESISTANCE; PROLIFERATION; INCREASES; APOPTOSIS; SURVIVIN; PLURIPOTENCY; EXPRESSION; ONCOGENE; GROWTH;

D O I：

10.3892/mmr.2014.2630

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Prostate cancer is the most commonly diagnosed type of cancer and the second leading cause of cancer-associated mortality in males. The efficacy of prostate cancer chemotherapy is frequently impaired by drug resistance; however, the underlying mechanisms of this resistance remain elusive. Sex determining region Y-box 2 (Sox2) is of vital importance in the regulation of stem cell proliferation and carcinogenesis. In the present study, using MTT, clone formation, cell cycle and apoptosis assays, over-expression of Sox2 was demonstrated to enhance the paclitaxel (Pac) resistance of the PC-3 prostate cancer cell line, promoting cell proliferation and exhibiting an anti-apoptotic effect. Western blot analysis revealed that the phosphoinositide 3-kinase/Akt signaling pathway was activated in cells overexpressing Sox2, and by targeting cyclin E and survivin, Sox2 promoted G1/S phase transition and prevented apoptosis under Pac treatment. The present study provided an understanding of Pac resistance in prostate cancer and may indicate novel therapeutic methods for chemoresistant prostate cancer.

引用

页码：3169 / 3176

页数：8

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