Aldose reductase inhibitor Epalrestat alleviates high glucose-induced cardiomyocyte apoptosis via ROS

被引：10
作者：
Wang, X. [1 ]
Yu, F. [2 ]
Zheng, W. -Q. [3 ]
机构：
[1] Weihai Cent Hosp, Dept Pharm, Weihai, Peoples R China
[2] Weihai Cent Hosp, Dept Dermatol, Weihai, Peoples R China
[3] Weihai Cent Hosp, Dept Cardiol, Weihai, Peoples R China
来源：
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES | 2019年 / 23卷
关键词：
Aldose reductase inhibitor; High blood glucose; Apoptosis; ROS; INFLAMMATION;
D O I：
10.26355/eurrev_201908_18660
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
OBJECTIVE: To clarify the role of aldose reductase inhibitor (ARI) in the high glucose-induced cardiomyocyte apoptosis and its mechanism. MATERIALS AND METHODS: In this study, H9c2 cardiomyocytes were employed as objects, high-glucose medium as stimulus, and ARI Epalrestat as a therapeutic drug. The cell apoptosis and activity changes of nitric oxide synthase (NOS), NO, and reactive oxygen species (ROS) were evaluated via Hoechst staining, enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), and Western blotting. In addition, the mitochondrial membrane potential was measured via fluorescence counting. RESULTS: Epalrestat inhibited the activity of AR to improve high glucose-induced oxidative stress in cardiomyocytes, weaken ROS activity, relieve the inhibition on NO activity, alleviate mitochondrial membrane potential damage, reduce the level of high glucose-induced cardiomyocyte apoptosis, and suppress the expression and activity of Caspase-3, thereby preventing high glucose-induced cardiomyocyte apoptosis. CONCLUSIONS: ARI protects against high glucose-induced cardiomyocyte apoptosis.
引用
收藏
页码：294 / 303
页数：10
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