MZF1 Transcriptionally Activated MicroRNA-328-3p Suppresses the Malignancy of Stomach Adenocarcinoma via Inhibiting CD44

被引:7
作者
Qi, Zining [1 ]
Wang, Jing [2 ]
Li, Yaoping [3 ]
Xu, Yanzhao [4 ]
机构
[1] Shanxi Med Univ, Hosp 1, Dept Gastrointestinal Surg, Taiyuan, Shanxi, Peoples R China
[2] Shanxi Canc Hosp, Dept Head & Neck Surg, Taiyuan, Shanxi, Peoples R China
[3] Shanxi Prov Peoples Hosp, Dept Colorectal Anorectal Surg, Taiyuan, Shanxi, Peoples R China
[4] Hebei Med Univ, Hosp 4, Dept Thorac Surg, Shijiazhuang, Hebei, Peoples R China
关键词
GASTRIC-CANCER;
D O I
10.1155/2022/5819295
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
MicroRNA-328-3p (miR-328-3p) plays a critical role in mediating the progression of multiple types of cancers. To date, no study has concentrated on the molecular mechanism of miR-328-3p in mediating stomach adenocarcinoma (STAD). In this study, it was found that miR-328-3p was downregulated in STAD, and inhibition of miR-328-3p significantly promoted the growth, migration, invasion, and stemness of STAD cells, while miR-328-3p overexpression exerted reverse effects. Through bioinformatics analysis, it was uncovered that a cluster of differentiation 44 (CD44) was upregulated in STAD and closely associated with the prognosis of STAD patients. Mechanistically, we identified CD44 as the target gene of miR-328-3p. Notably, knockdown of CD44 abolished the promoting function of miR-328-3p inhibitor in the development of STAD. Moreover, myeloid zinc finger protein 1 (MZF1) was confirmed as an upstream transcription factor for miR-328-3p, which is involved in enhancing miR-328-3p expression. In addition, the role of MZF1 downregulation in the malignant traits of STAD cells was blocked by miR-328-3p overexpression. More importantly, upregulation of miR-328-3p efficiently suppressed STAD tumor growth in vivo. Collectively, our findings illustrated that MZF1-mediated miR-328-3p acted as a cancer suppressor in STAD progression via regulation of CD44, which suggested the possibility of the MZF1/miR-328-3p/CD44 axis as a novel promising therapeutic candidate for STAD.
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页数:17
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