Optimization of large-ring cyclodextrin production from starch by amylomaltase from Corynebacterium glutamicum and effect of organic solvent on product size

被引：16
作者：
Vongpichayapaiboon, T. [1 ]
Pongsawasdi, P. [1 ]
Krusong, K. [1 ]
机构：
[1] Chulalongkorn Univ, Fac Sci, Dept Biochem, Starch & Cyclodextrin Res Unit, Bangkok 10330, Thailand
来源：
JOURNAL OF APPLIED MICROBIOLOGY | 2016年 / 120卷 / 04期
关键词：
amylomaltase; Corynebacterium glutamicum; cyclization; cycloamylose; large-ring cyclodextrins; LR-CDs; POTATO D-ENZYME; COMPLEX-FORMATION; DELIVERY-SYSTEM; CYCLOAMYLOSE; GLYCOSYLTRANSFERASE; AMYLOSE; CYCLIZATION; GLUCANOTRANSFERASES; IMPROVE; CGTASE;
D O I：
10.1111/jam.13087
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
AimsTo increase yield of starch conversion to large-ring cyclodextrins (LR-CDs) by amylomaltase from Corynebacterium glutamicum (CgAM). Methods and ResultsIn this work, LR-CDs produced from pea, tapioca, corn, potato, rice and glutinous-rice starch by the recombinant CgAM were analysed by High-Performance Anion-Exchange Chromatography Using Pulsed Amperometric Detection (HPAEC-PAD). Among these, pea starch gave the highest yield of LR-CDs. Pretreatment of pea starch with isoamylase prior to incubation with CgAM resulted in the increase in LR-CD products by 20%. Surprisingly, CgAM converted starch into LR-CDs within a wide pH range (pH 55-90). LR-CD syntheses at alkaline pH or at a long incubation time favoured low-degree of polymerization (DP) products (CD22-CD32). Addition of 5-15% dimethyl sulfoxide (DMSO) promoted the synthesis of medium-DP species (CD33-CD43) by 10-25%. ConclusionsPretreatment of pea starch with isoamylase could enhance the yield of LR-CDs. The ratio of LR-CD products depends on pH, incubation time and addition of organic solvents such as DMSO. Significance and Impact of the StudyLR-CD yield can be increased by thorough optimization of starch types, starch concentrations, enzyme activities, pH and incubation times. This study is the first report of the effect of organic solvents on LR-CD production by amylomaltase.
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收藏
页码：912 / 920
页数：9
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