Degradation of Microcystin-LR and RR by a Stenotrophomonas sp Strain EMS Isolated from Lake Taihu, China

被引：80

作者：

Chen, Jian ^{[1
,3
]}

Hu, Liang Bin ^{[2
]}

Zhou, Wei ^{[1
,3
]}

Yan, Shao Hua ^{[3
,4
]}

Yang, Jing Dong ^{[1
,3
]}

Xue, Yan Feng ^{[1
,3
]}

Shi, Zhi Qi ^{[1
,3
]}

机构：

[1] Jiangsu Acad Agr Sci, Inst Food Safety & Qual, Nanjing 210014, Peoples R China

[2] Henan Inst Sci & Technol, Sch Food, Xinxiang 453003, Peoples R China

[3] Minist Agr, Key Lab Food Safety Monitoring & Management, Nanjing 210014, Peoples R China

[4] Jiangsu Acad Agr Sci, Inst Resources & Environm Sci, Nanjing 210014, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2010年 / 11卷 / 03期

基金：

中国国家自然科学基金;

关键词：

degradation; microcystin-LR; microcystin-RR; cyanobacteria; EMS; mlrA; BACTERIAL-DEGRADATION; CYANOBACTERIAL TOXIN; WATER; BIODEGRADATION; DNA; EUTROPHICATION; PEPTIDES; GROWTH;

D O I：

10.3390/ijms11030896

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A bacterial strain EMS with the capability of degrading microcystins (MCs) was isolated from Lake Taihu, China. The bacterium was tentatively identified as a Stenotrophomonas sp. The bacterium could completely consume MC-LR and MC-RR within 24 hours at a concentration of 0.7 mu g/mL and 1.7 mu g/mL, respectively. The degradation of MC-LR and MC-RR by EMS occurred preferentially in an alkaline environment. In addition, mlrA gene involved in the degradation of MC-LR and MC-RR was detected in EMS. Due to the limited literature this gene has rare homologues. Sequencing analysis of the translated protein from mlrA suggested that MlrA might be a transmembrane protein, which suggests a possible new protease family having unique function.

引用

页码：896 / 911

页数：16

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