Rheology of gastric mucin exhibits a pH-dependent sol-gel transition

被引：256
作者：
Celli, Jonathan P.
Turner, Bradley S.
Afdhal, Nezam H.
Ewoldt, Randy H.
McKinley, Gareth H.
Bansil, Rama
Erramilli, Shyamsunder
机构：
[1] Boston Univ, Dept Phys, Boston, MA 02215 USA
[2] Beth Israel Deaconess Med Ctr, Boston, MA 02215 USA
[3] Harvard Univ, Sch Med, Boston, MA 02115 USA
[4] MIT, Dept Mech Engn, Hatsopoulos Microfluids Lab, Cambridge, MA 02139 USA
来源：
BIOMACROMOLECULES | 2007年 / 8卷 / 05期
关键词：
D O I：
10.1021/bm0609691
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Gastric mucin, a high molecular weight glycoprotein, is responsible for providing the gel-forming properties and protective function of the gastric mucus layer. Bulk rheology measurements in the linear viscoelastic regime show that gastric mucin undergoes a pH-dependent sol-gel transition from a viscoelastic solution at neutral pH to a soft viscoelastic gel in acidic conditions, with the transition occurring near pH 4. In addition to pH-dependent gelation behavior in this system, further rheological studies under nonlinear deformations reveal shear thinning and an apparent yield stress in this material which are also highly influenced by pH.
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页码：1580 / 1586
页数：7
相关论文
共 53 条
[1]   THE MUCUS BARRIER - ITS ROLE IN GASTRODUODENAL MUCOSAL PROTECTION [J].
ALLEN, A ;
LEONARD, AJ ;
SELLERS, LA .
JOURNAL OF CLINICAL GASTROENTEROLOGY, 1988, 10 :S93-S98
[2]  
Allen A., 1981, PHYSL GASTROINTESTIN, P617
[3]  
Allen P M, 1988, FEMS Microbiol Immunol, V1, P19
[4]   The adherent gastrointestinal mucus gel layer: thickness and physical state in vivo [J].
Atuma, C ;
Strugala, V ;
Allen, A ;
Holm, L .
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2001, 280 (05) :G922-G929
[5]  
BANSIL R, 2005, MRS 2005 FALL P S, V897
[6]   Mucin structure, aggregation, physiological functions and biomedical applications [J].
Bansil, Rama ;
Turner, Bradley S. .
CURRENT OPINION IN COLLOID & INTERFACE SCIENCE, 2006, 11 (2-3) :164-170
[7]   The yield stress -: a review or 'παντα ρει' -: everything flows? [J].
Barnes, HA .
JOURNAL OF NON-NEWTONIAN FLUID MECHANICS, 1999, 81 (1-2) :133-178
[8]   Regulated alkali secretion acts in tandem with unstirred layers to regulate mouse gastric surface pH [J].
Baumgartner, HK ;
Montrose, MH .
GASTROENTEROLOGY, 2004, 126 (03) :774-783
[9]   PROPERTIES OF GASTRIC AND DUODENAL MUCUS - EFFECT OF PROTEOLYSIS, DISULFIDE REDUCTION, BILE, ACID, ETHANOL, AND HYPERTONICITY ON MUCUS GEL STRUCTURE [J].
BELL, AE ;
SELLERS, LA ;
ALLEN, A ;
CUNLIFFE, WJ ;
MORRIS, ER ;
ROSSMURPHY, SB .
GASTROENTEROLOGY, 1985, 88 (01) :269-280
[10]   PROFOUND INCREASE IN VISCOSITY AND AGGREGATION OF PIG GASTRIC MUCIN AT LOW PH [J].
BHASKAR, KR ;
GONG, D ;
BANSIL, R ;
PAJEVIC, S ;
HAMILTON, JA ;
TURNER, BS ;
LAMONT, JT .
AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 261 (05) :G827-G833
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