Identification of the bovine HSL gene expression profiles and its association with fatty acid composition and fat deposition traits

被引：33

作者：

Fang, Xibi ^{[1
]}

Zhao, Zhihui ^{[1
]}

Jiang, Ping ^{[1
]}

Yu, Haibin ^{[1
]}

Xiao, Hang ^{[1
]}

Yang, Runjun ^{[1
]}

机构：

[1] Jilin Univ, Coll Anim Sci, 5333 Xian Rd, Changchun 130062, Peoples R China

来源：

MEAT SCIENCE | 2017年 / 131卷

基金：

中国国家自然科学基金;

关键词：

HSL; Lipometabolism; Gene promoter; Beef cattle; Fatty acid composition; HORMONE-SENSITIVE LIPASE; C/EBP-ALPHA; NUTRITIONAL COMPOSITION; BINDING-PROTEINS; ADIPOSE-TISSUE; BEEF-CATTLE; TRANSCRIPTION; GROWTH; CELLS; HMGA1;

D O I：

10.1016/j.meatsci.2017.05.003

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Hormone-sensitive lipase (HSL) is an intracellular neutral lipase capable of hydrolysing a variety of esters and is considered to be a candidate gene affecting fat deposition traits. Gene expression profiles of HSL were analysed in various adipose tissues of cattle, and the effect of HSL on lipid metabolism genes was analysed by a PCR array. Novel polymorphisms were identified within the HSL regulatory domain by sequencing, and the relationship between HSL variants and fat deposition traits was analysed. HSL mRNA was highly expressed in the subcutaneous and visceral fat of cattle. CPT1B/CPT1C and other lipocatabolic genes were upregulated, and lipogenesis-related genes (FASN, LPL and ACOT12) were downregulated by HSL overexpression in BFFs. Five novel variants in the HSL functional domain were significantly associated with fat deposition traits, including FCR, LBT, MFW and fatty acid composition. HSL plays a pivotal role in the regulation of lipolysis and fatty acid biosynthesis in beef cattle.

引用

页码：107 / 118

页数：12

共 37 条

[21] FUNCTIONS OF FATTY-ACID BINDING-PROTEINS [J].

KAIKAUS, RM ;

BASS, NM ;

OCKNER, RK .

EXPERIENTIA, 1990, 46 (06) :617-630

[22] Association of melanocortin 4 receptor (MC4R) and high mobility group AT-hook 1 (HMGA1) polymorphisms with pig growth and fat deposition traits [J].

Kim, K. S. ;

Lee, J. J. ;

Shin, H. Y. ;

Choi, B. H. ;

Lee, C. K. ;

Kim, J. J. ;

Cho, B. W. ;

Kim, T. -H. .

ANIMAL GENETICS, 2006, 37 (04) :419-421

[23] Hormone-sensitive lipase: control of intracellular tri-(di-)acylglycerol and cholesteryl ester hydrolysis [J].

Kraemer, FB ;

Shen, WJ .

JOURNAL OF LIPID RESEARCH, 2002, 43 (10) :1585-1594

[24]

Laborde FL, 2001, J ANIM SCI, V79, P355

[25] HMG1A and PPARG are differently expressed in the liver of fat and lean broilers [J].

Larkina, Tatiana A. ;

Sazanova, Anna L. ;

Fomichev, Kirill A. ;

Barkova, Olga Y. ;

Malewski, Tadeusz ;

Jaszczak, Kazimierz ;

Sazanov, Alexei A. .

JOURNAL OF APPLIED GENETICS, 2011, 52 (02) :225-228

[26] Transcription of eukaryotic protein-coding genes [J].

Lee, TI ;

Young, RA .

ANNUAL REVIEW OF GENETICS, 2000, 34 :77-137

[27] C/EBPα is required for differentiation of white, but not brown, adipose tissue [J].

Linhart, HG ;

Ishimura-Oka, K ;

DeMayo, F ;

Kibe, T ;

Repka, D ;

Poindexter, B ;

Bick, RJ ;

Darlington, GJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (22) :12532-12537

[28] Comparison of the lipid content and fatty acid composition of intermuscular and subcutaneous adipose tissues in pig carcasses [J].

Monziois, M. ;

Bonneau, M. ;

Davenel, A. ;

Kouba, M. .

MEAT SCIENCE, 2007, 76 (01) :54-60

[29] Genetic variation at the hormone sensitive lipase: gender-specific association with plasma lipid and glucose concentrations [J].

Qi, L ;

Shen, H ;

Larson, I ;

Barnard, JR ;

Schaefer, EJ ;

Ordovas, JM .

CLINICAL GENETICS, 2004, 65 (02) :93-100

[30]

Shawlot W, 1999, DEVELOPMENT, V126, P4925

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