Identification of the bovine HSL gene expression profiles and its association with fatty acid composition and fat deposition traits

被引：32
作者：
Fang, Xibi [1 ]
Zhao, Zhihui [1 ]
Jiang, Ping [1 ]
Yu, Haibin [1 ]
Xiao, Hang [1 ]
Yang, Runjun [1 ]
机构：
[1] Jilin Univ, Coll Anim Sci, 5333 Xian Rd, Changchun 130062, Peoples R China
来源：
MEAT SCIENCE | 2017年 / 131卷
基金：
中国国家自然科学基金;
关键词：
HSL; Lipometabolism; Gene promoter; Beef cattle; Fatty acid composition; HORMONE-SENSITIVE LIPASE; C/EBP-ALPHA; NUTRITIONAL COMPOSITION; BINDING-PROTEINS; ADIPOSE-TISSUE; BEEF-CATTLE; TRANSCRIPTION; GROWTH; CELLS; HMGA1;
D O I：
10.1016/j.meatsci.2017.05.003
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Hormone-sensitive lipase (HSL) is an intracellular neutral lipase capable of hydrolysing a variety of esters and is considered to be a candidate gene affecting fat deposition traits. Gene expression profiles of HSL were analysed in various adipose tissues of cattle, and the effect of HSL on lipid metabolism genes was analysed by a PCR array. Novel polymorphisms were identified within the HSL regulatory domain by sequencing, and the relationship between HSL variants and fat deposition traits was analysed. HSL mRNA was highly expressed in the subcutaneous and visceral fat of cattle. CPT1B/CPT1C and other lipocatabolic genes were upregulated, and lipogenesis-related genes (FASN, LPL and ACOT12) were downregulated by HSL overexpression in BFFs. Five novel variants in the HSL functional domain were significantly associated with fat deposition traits, including FCR, LBT, MFW and fatty acid composition. HSL plays a pivotal role in the regulation of lipolysis and fatty acid biosynthesis in beef cattle.
引用
收藏
页码：107 / 118
页数：12
相关论文
共 37 条
  • [1] Novel Mutations of the FASN Gene and Their Effect on Fatty Acid Composition in Japanese Black Beef
    Abe, Tsuyoshi
    Saburi, Junich
    Hasebe, Hiroyuki
    Nakagawa, Tetsuo
    Misumi, Satsuki
    Nade, Toshihiro
    Nakajima, Hiroaki
    Shoji, Noriaki
    Kobayashi, Masato
    Kobayashi, Eiji
    [J]. BIOCHEMICAL GENETICS, 2009, 47 (5-6) : 397 - 411
  • [2] C/EBPα gene as a genetic marker for beef quality improvement
    Adoligbe, C.
    Huangfu, Y. F.
    Zan, L. S.
    Wang, H.
    [J]. GENETICS AND MOLECULAR RESEARCH, 2015, 14 (03) : 9370 - 9383
  • [3] HMGA1 overexpression in adipose tissue impairs adipogenesis and prevents diet-induced obesity and insulin resistance
    Arce-Cerezo, Altamira
    Garcia, Miquel
    Rodriguez-Nuevo, Aida
    Crosa-Bonell, Mireia
    Enguix, Natalia
    Pero, Albert
    Munoz, Sergio
    Roca, Carles
    Ramos, David
    Franckhauser, Sylvie
    Elias, Ivet
    Ferre, Tura
    Pujol, Anna
    Ruberte, Jesus
    Villena, Josep A.
    Bosch, Fatima
    Riu, Efren
    [J]. SCIENTIFIC REPORTS, 2015, 5
  • [4] Bonnefont Jean-Paul, 2004, Molecular Aspects of Medicine, V25, P495, DOI 10.1016/j.mam.2004.06.004
  • [5] BOTSTEIN D, 1980, AM J HUM GENET, V32, P314
  • [6] Chen X. L., 2016, PLOS ONE, V11
  • [7] Animal fatty acid synthase: Functional mapping and cloning and expression of the domain I constituent activities
    Chirala, SS
    Huang, WY
    Jayakumar, A
    Sakai, K
    Wakil, SJ
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (11) : 5588 - 5593
  • [8] The Minimal Domain of Adipose Triglyceride Lipase (ATGL) Ranges until Leucine 254 and Can Be Activated and Inhibited by CGI-58 and G0S2, Respectively
    Cornaciu, Irina
    Boeszoermenyi, Andras
    Lindermuth, Hanna
    Nagy, Harald M.
    Cerk, Ines K.
    Ebner, Catharina
    Salzburger, Barbara
    Gruber, Astrid
    Schweiger, Martina
    Zechner, Rudolf
    Lass, Achim
    Zimmermann, Robert
    Oberer, Monika
    [J]. PLOS ONE, 2011, 6 (10):
  • [9] Daval S, 2000, GENET SEL EVOL, V32, P521, DOI 10.1051/gse:2000134
  • [10] Meat nutritional composition and nutritive role in the human diet
    de Castro Cardoso Pereira, Paula Manuela
    dos Reis Baltazar Vicente, Ana Filipa
    [J]. MEAT SCIENCE, 2013, 93 (03) : 586 - 592
1234