Efficacy of Chemical Treatments Against Murine Norovirus, Feline Calicivirus, and MS2 Bacteriophage

被引:71
作者
D'Souza, Doris H. [1 ]
Su, Xiaowei [1 ]
机构
[1] Univ Tennessee, Dept Food Sci & Technol, Knoxville, TN 37996 USA
关键词
ESCHERICHIA-COLI O157-H7; HEPATITIS-A VIRUS; ENTERIC VIRUSES; DISINFECTION; INACTIVATION; SURROGATE; SURFACE; RESISTANCE; ETHANOL; MODEL;
D O I
10.1089/fpd.2009.0426
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Human noroviruses pose an emerging public health threat, and despite stringent control strategies, variant strains continue to spread and cause disease outbreaks. Routinely used chemical sanitizers, such as sodium hypochlorite though effective on food contact surfaces, require high concentrations to cause reduction in enteric viral titers. The aim of this study was to evaluate the efficacy of trisodium phosphate (TSP) against three human enteric virus surrogates, murine norovirus (MNV-1), feline calicivirus (FCV), and bacteriophage MS2 in comparison to routinely used sanitizers. Three concentrations of TSP (1%, 2%, and 5%) at different contact times (30 sec and 1 min) were evaluated against the surrogate viruses individually inoculated on formica coupons. Our results showed that 5% TSP was effective in obtaining a >= 6 log(10) PFU reduction for MNV-1, FCV, and MS2 after a contact time of only 30 sec or 1 min similar to 10% household bleach (0.6% sodium hypochlorite, 5000ppm available chlorine) for high titers of FCV and MS2, and with similar to 5 log(10) reduction after either 30 sec or 1 min on low viral titers. However, 2% TSP for 1 min resulted in similar to 6 log(10) PFU reduction for FCV and MS2, but only a 1.05 log(10) PFU reduction for MNV-1 at high titers, with similar results after 30 sec. Decreasing TSP levels to 1% reduced FCV by similar to 2.65 log(10) PFU, MS2 by 4.5 log(10) PFU at high titers, and no reduction for MNV-1 after 30-sec or 1 min contact. Glutaraldehyde (2%) reduced FCV and MNV-1 titers by similar to 6 log(10) PFU; however, MS2 was reduced by only 3.22 and 3.74 log(10) PFU after 30 sec and 1 min, respectively, while 70% ethanol was not effective in reducing the three viruses at either high or low titers at both contact times. Alternative control strategies using TSP with short contact times should benefit the food industry in reducing norovirus transmission.
引用
收藏
页码:319 / 326
页数:8
相关论文
共 32 条
[11]   Inactivation of Norovirus by ozone gas in conditions relevant to healthcare [J].
Hudson, J. B. ;
Sharma, M. ;
Petric, M. .
JOURNAL OF HOSPITAL INFECTION, 2007, 66 (01) :40-45
[12]   Effectiveness of commercial disinfectants for inactivating hepatitis A virus on agri-food surfaces [J].
Jean, J ;
Vachon, JF ;
Moroni, O ;
Darveau, A ;
Kukavica-Ibrulj, I ;
Fliss, I .
JOURNAL OF FOOD PROTECTION, 2003, 66 (01) :115-119
[13]   Efficacy of three ethanol-based hand rubs against feline calicivirus, a surrogate virus for norovirus [J].
Kampf, G ;
Grotheer, D ;
Steinmann, J .
JOURNAL OF HOSPITAL INFECTION, 2005, 60 (02) :144-149
[14]   STAT1-dependent innate immunity to a Norwalk-like virus [J].
Karst, SM ;
Wobus, CE ;
Lay, M ;
Davidson, J ;
Virgin, HW .
SCIENCE, 2003, 299 (5612) :1575-1578
[15]   In-vivo efficacy of hand sanitisers against feline calicivirus: a surrogate for norovirus [J].
Lages, S. L. S. ;
Ramakrishnan, M. A. ;
Goyal, S. M. .
JOURNAL OF HOSPITAL INFECTION, 2008, 68 (02) :159-163
[16]   CHEMICAL DISINFECTION OF HUMAN ROTAVIRUS-CONTAMINATED INANIMATE SURFACES [J].
LLOYDEVANS, N ;
SPRINGTHORPE, VS ;
SATTAR, SA .
JOURNAL OF HYGIENE, 1986, 97 (01) :163-173
[17]   Reduction of poliovirus 1, bacteriophages, Salmonella Montevideo, and Escherichia coli O157: H7 on strawberries by physical and disinfectant washes [J].
Lukasik, J ;
Bradley, ML ;
Scott, TM ;
Dea, M ;
Koo, A ;
Hsu, WY ;
Bartz, JA ;
Farrah, SR .
JOURNAL OF FOOD PROTECTION, 2003, 66 (02) :188-193
[18]   Improved inactivation of nonenveloped enteric viruses and their surrogates by a novel alcohol-based hand sanitizer [J].
Macinga, David R. ;
Sattar, Syed A. ;
Jaykus, Lee-Ann ;
Arbogast, James W. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (16) :5047-5052
[19]   EFFECT OF BIOCIDES ON MS2-COLIPHAGE AND K-COLIPHAGE [J].
MAILLARD, JY ;
BEGGS, TS ;
DAY, MJ ;
HUDSON, RA ;
RUSSELL, AD .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (06) :2205-2206
[20]   Virucidal efficacy of sodium bicarbonate on a food contact surface against feline calicivirus, a norovirus surrogate [J].
Malik, Yashpal S. ;
Goyal, Sagar M. .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2006, 109 (1-2) :160-163