Giant crystals inside mitochondria of equine chondrocytes

被引:6
作者
Nuernberger, S. [1 ,2 ,3 ,4 ]
Rentenberger, C. [5 ]
Thiel, K. [6 ]
Schaedl, B. [2 ,3 ,4 ]
Grunwald, I. [6 ]
Ponomarev, I. [9 ]
Marlovits, St [1 ,4 ]
Meyer, Ch. [7 ,8 ]
Barnewitz, D. [9 ]
机构
[1] Med Univ Vienna, Dept Trauma Surg, Waehringer Guertel 18-20, A-1090 Vienna, Austria
[2] AUVA Res Ctr, Ludwig Boltzmann Inst Expt & Clin Traumatol, Donaueschingenstr 13, A-1200 Vienna, Austria
[3] Med Univ Vienna, Univ Clin Dent, Sensengasse 2a, Vienna, Austria
[4] Austrian Cluster Tissue Regenerat, Vienna, Austria
[5] Univ Vienna, Phys Nanostruct Mat, Fac Phys, Boltzmanngasse 5, A-1090 Vienna, Austria
[6] Fraunhofer Inst Mfg Technol & Adv Mat, Dept Adhes Bonding Technol & Surfaces, Wiener Str 12, D-28359 Bremen, Germany
[7] Univ Giessen, Clin & Polyclin Traumatol, Rudolf Buchheim Str 7, D-35385 Giessen, Germany
[8] Klinikum Saarbrucken, Orthopaed & Trauma Surg, Winterberg 1, D-66119 Saarbrucken, Germany
[9] Res Ctr Med Tech & Biotechnol, Geranienweg 7, D-99947 Bad Langensalza, Germany
关键词
Cartilage; Protein crystals; Mitochondria; Chondrocytes; Histochemistry; Transmission electron microscopy; PARACRYSTALLINE INCLUSIONS; SUBMICROSCOPIC STRUCTURE; STORING HISTIOCYTOSIS; ELECTRON-MICROSCOPE; LIVER-CELLS; INTRAMITOCHONDRIAL; PROTEIN; CRYSTALLIZATION; ULTRASTRUCTURE; CARTILAGE;
D O I
10.1007/s00418-016-1516-6
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The present study reports for the first time the presence of giant crystals in mitochondria of equine chondrocytes. These structures show dark contrast in TEM images as well as a granular substructure of regularly aligned 1-2 nm small units. Different zone axes of the crystalline structure were analysed by means of Fourier transformation of lattice-resolution TEM images proving the crystalline nature of the structure. Elemental analysis reveals a high content of nitrogen referring to protein. The outer shape of the crystals is geometrical with an up to hexagonal profile in cross sections. It is elongated, spanning a length of several micrometres through the whole cell. In some chondrocytes, several crystals were found, sometimes combined in a single mitochondrion. Crystals were preferentially aligned along the long axis of the cells, thus appearing in the same orientation as the chondrocytes in the tissue. Although no similar structures have been found in the cartilage of any other species investigated, they have been found in cartilage repair tissue formed within a mechanically stimulated equine chondrocyte construct. Crystals were mainly located in superficial regions of cartilage, especially in joint regions of well-developed superficial layers, more often in yearlings than in adult horses. These results indicate that intramitochondrial crystals are related to the high mechanical stress in the horse joint and potentially also to the increased metabolic activity of immature individuals.
引用
收藏
页码:635 / 649
页数:15
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