Improved determination of urinary cortisol and cortisone, or corticosterone and 11-dehydrocorticosterone by high-performance liquid chromatography with ultraviolet absorbance detection

被引：74

作者：

Hay, M ^{[1
]}

Mormède, P ^{[1
]}

机构：

[1] Inst Francois Magendie, INRA, INSERM, Lab Genet Stress, F-33077 Bordeaux, France

来源：

JOURNAL OF CHROMATOGRAPHY B | 1997年 / 702卷 / 1-2期

关键词：

cortisol; cortisone; corticosterone; 11-dehydrocorticosterone;

D O I：

10.1016/S0378-4347(97)00361-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive assay was developed for the determination of low levels of free (unconjugated) glucocorticoids in human, swine (cortisol and cortisone) and rat urine (corticosterone and 11-dehydrocorticosterone), using solid-phase extraction and HPLC with UV absorbance detection (254 nm). Precise quantitation is allowed by the use of internal standards (dexamethasone for swine urine and Reichstein's substance S for rat urine). This simple method allows the use of small urine samples (less than 2 ml), and is suitable for a wide range of applications in human and animal clinical and physiological studies. (C) 1997 Elsevier Science B.V.

引用

页码：33 / 39

页数：7

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