A food-grade engineered Lactococcus lactis strain delivering Helicobacter pylori Lpp20 alleviates bacterial infection in H. pylori-challenged mice

被引：10

作者：

Sun, Nan ^{[1
]}

Zhang, Rongguang ^{[1
]}

Duan, Guangcai ^{[1
]}

Peng, Xiaoyan ^{[1
]}

Wang, Chen ^{[1
]}

Chen, Shuaiyin ^{[1
]}

Fan, Qingtang ^{[1
]}

机构：

[1] Zhengzhou Univ, Coll Publ Hlth, Dept Epidemiol & Stat, 100 Kexue Ave, Zhengzhou 450001, Henan, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2019年 / 41卷 / 12期

基金：

中国国家自然科学基金;

关键词：

Food-grade bacterium; Functional food; Genetically engineering; Helicobacter pylori; Lactococcus lactis; Oral vaccination; ORAL IMMUNIZATION; EXPRESSION; SUBUNIT; SYSTEM; GENE;

D O I：

10.1007/s10529-019-02740-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Objective To construct a food-grade bacterium producing and delivering H. pylori Lpp20 antigen and evaluate its immune efficacy against H. pylori challenges with aim to develop anti-H. pylori oral vaccines and functional foods. Results Lpp20 was expressed as a 22 kDa protein in Lactococcus lactis, constituting 11.2% of the cell lysate proteins, and recognized by mouse antisera. Mice orally gavaged with the engineered bacterium had elevated serum IgG levels and lowered urease activity of stomach following H. pylori challenges. Conclusions This study firstly reports a food-grade L. lactis strain delivering Lpp20 to mucosal immunization sites, demonstrating a novel efficient production and safe utilization mode of Lpp20, offering a promising vaccine candidate and health food sources.

引用

页码：1415 / 1421

页数：7

共 13 条

[1]

Abdoh Qusay, 2018, Lancet, V391 Suppl 2, pS32, DOI 10.1016/S0140-6736(18)30398-2

[2] Food-Grade Expression of Helicobacter pylori UreB Subunit in Lactococcus lactis and its Immunoreactivity [J].

Chen, Shuaiyin ;

Zhang, Rongguang ;

Duan, Guangcai ;

Shi, Jianxiang .

CURRENT MICROBIOLOGY, 2011, 62 (06) :1726-1731

[3] Oral vaccination of mice against Helicobacter pylori with recombinant Lactococcus lactis expressing urease subunit B [J].

Gu, Qing ;

Song, Dafeng ;

Zhu, Muyuan .

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2009, 56 (03) :197-203

[4] Expression of Helicobacter pylori urease subunit B gene in Lactococcus lactis MG1363 and its use as a vaccine delivery system against H-pylori infection in mice [J].

Lee, MH ;

Roussel, Y ;

Wilks, M ;

Tabaqchali, S .

VACCINE, 2001, 19 (28-29) :3927-3935

[5] Oral immunization with recombinant Lactococcus lactis delivering a multi-epitope antigen CTB-UE attenuates Helicobacter pylori infection in mice [J].

Li, Xinyang ;

Xing, Yingying ;

Guo, Le ;

Lv, Xiaobo ;

Song, Hui ;

Xi, Tao .

PATHOGENS AND DISEASE, 2014, 72 (01) :78-86

[6] Antibody Production and Th1-biased Response Induced by an Epitope Vaccine Composed of Cholera Toxin B Unit and Helicobacter pylori Lpp20 Epitopes [J].

Li, Yan ;

Chen, Zhongbiao ;

Ye, Jianbin ;

Ning, Lijun ;

Luo, Jun ;

Zhang, Lili ;

Jiang, Yin ;

Xi, Yue ;

Ning, Yunshan .

HELICOBACTER, 2016, 21 (03) :234-248

[7] Therapeutic efficacy of oral immunization with a non-genetically modified Lactococcus lactis-based vaccine CUE-GEM induces local immunity against Helicobacter pylori infection [J].

Liu, Wei ;

Tan, Zhoulin ;

Xue, Jinfeng ;

Luo, Wenjin ;

Song, Hui ;

Lv, Xiaobo ;

Zheng, Tianjing ;

Xi, Tao ;

Xing, Yingying .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 100 (14) :6219-6229

[8] Mutation of iceA in Helicobacter pylori compromised IL-8 induction from human gastric epithelial cells [J].

Ma, Yi Jun ;

Duan, Guang Cai ;

Zhang, Rong Guang ;

Fan, Qing Tang ;

Zhang, Wei Dong .

JOURNAL OF BASIC MICROBIOLOGY, 2010, 50 :S83-S88

[9] Production and delivery of Helicobacter pylori NapA in Lactococcus lactis and its protective efficacy and immune modulatory activity [J].

Peng, Xiaoyan ;

Zhang, Rongguang ;

Duan, Guangcai ;

Wang, Chen ;

Sun, Nan ;

Zhang, Linghan ;

Chen, Shuaiyin ;

Fan, Qingtang ;

Xi, Yuanlin .

SCIENTIFIC REPORTS, 2018, 8

[10] An engineered food-grade Lactococcus lactis strain for production and delivery of heat-labile enterotoxin B subunit to mucosal sites [J].

Sun, Nan ;

Zhang, Rongguang ;

Duan, Guangcai ;

Peng, Xiaoyan ;

Wang, Chen ;

Fan, Qingtang ;

Chen, Shuaiyin ;

Xi, Yuanlin .

BMC BIOTECHNOLOGY, 2017, 17

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