Analysis and overexpression in Escherichia coli of a staphylococcal gene encoding seryl-tRNA synthetase

被引：1

作者：

Bausch, N ^{[1
]}

Seignovert, L ^{[1
]}

Beaulande, M ^{[1
]}

Leberman, R ^{[1
]}

Härtlein, M ^{[1
]}

机构：

[1] European Mol Biol Lab, Grenoble Outstn, F-38042 Grenoble, France

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1998年 / 1397卷 / 02期

关键词：

aminoacyl-tRNA synthetase; calmodulin-binding peptide expression system; design of antibiotics; (Staphylococcus aureus);

D O I：

10.1016/S0167-4781(98)00027-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have sequenced and expressed in Escherichia coli the gene encoding the seryl-tRNA synthetase from the pathogenic bacterium Staphylococcus aureus. The overexpressed and purified recombinant enzyme was able to aminoacylate unfractionated tRNA from E. coli. Its activity was not affected by antibodies raised against and inhibiting the E. coli seryl-tRNA synthetase. (C) 1998 Elsevier Science B.V.

引用

页码：169 / 174

页数：6

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