Analysis and overexpression in Escherichia coli of a staphylococcal gene encoding seryl-tRNA synthetase

被引：1

作者：

Bausch, N ^{[1
]}

Seignovert, L ^{[1
]}

Beaulande, M ^{[1
]}

Leberman, R ^{[1
]}

Härtlein, M ^{[1
]}

机构：

[1] European Mol Biol Lab, Grenoble Outstn, F-38042 Grenoble, France

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1998年 / 1397卷 / 02期

关键词：

aminoacyl-tRNA synthetase; calmodulin-binding peptide expression system; design of antibiotics; (Staphylococcus aureus);

D O I：

10.1016/S0167-4781(98)00027-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have sequenced and expressed in Escherichia coli the gene encoding the seryl-tRNA synthetase from the pathogenic bacterium Staphylococcus aureus. The overexpressed and purified recombinant enzyme was able to aminoacylate unfractionated tRNA from E. coli. Its activity was not affected by antibodies raised against and inhibiting the E. coli seryl-tRNA synthetase. (C) 1998 Elsevier Science B.V.

引用

页码：169 / 174

页数：6

共 16 条

[1] OVERPRODUCTION OF TYROSYL-TRANSFER RNA-SYNTHETASE IS TOXIC TO ESCHERICHIA-COLI - A GENETIC-ANALYSIS
BEDOUELLE, H
GUEZ, V
VIDALCROS, A
HERMANN, M
[J]. JOURNAL OF BACTERIOLOGY, 1990, 172 (07) : 3940 - 3945
[2] THE 2.9 ANGSTROM CRYSTAL-STRUCTURE OF THERMUS-THERMOPHILUS SERYL-TRANSFER-RNA SYNTHETASE COMPLEXED WITH TRNA(SER)
BIOU, V
YAREMCHUK, A
TUKALO, M
CUSACK, S
[J]. SCIENCE, 1994, 263 (5152) : 1404 - 1410
[3] BOREL F, 1994, NUCLEIC ACIDS RES, V22, P963
[4] ANALYSIS AND TOXIC OVEREXPRESSION IN ESCHERICHIA-COLI OF A STAPHYLOCOCCAL GENE ENCODING ISOLEUCYL-TRANSFER-RNA SYNTHETASE
CHALKER, AF
WARD, JM
FOSBERRY, AP
HODGSON, JE
[J]. GENE, 1994, 141 (01) : 103 - 108
[5] The crystal structure of the ternary complex of T-thermophilus seryl-tRNA synthetase with tRNA(Ser) and a seryl-adenylate analogue reveals a conformational switch in the active site
Cusack, S
Yaremchuk, A
Tukalo, M
[J]. EMBO JOURNAL, 1996, 15 (11) : 2834 - 2842
[6] A 2ND CLASS OF SYNTHETASE STRUCTURE REVEALED BY X-RAY-ANALYSIS OF ESCHERICHIA-COLI SERYL-TRANSFER RNA-SYNTHETASE AT 2.5-A
CUSACK, S
BERTHETCOLOMINAS, C
HARTLEIN, M
NASSAR, N
LEBERMAN, R
[J]. NATURE, 1990, 347 (6290) : 249 - 255
[7] HIGH-LEVEL MUPIROCIN RESISTANCE IN STAPHYLOCOCCUS-AUREUS - EVIDENCE FOR 2 DISTINCT ISOLEUCYL-TRANSFER RNA-SYNTHETASES
GILBART, J
PERRY, CR
SLOCOMBE, B
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1993, 37 (01) : 32 - 38
[8] Grundy Frank J., 1997, Journal of Bacteriology, V179, P3767
[9] Species-specific tRNA recognition in relation to tRNA synthetase contact residues
Nair, S
dePouplana, LR
Houman, F
Avruch, A
Shen, XY
Schimmel, P
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1997, 269 (01) : 1 - 9
[10] Variant minihelix RNAs reveal sequence-specific recognition of the helical tRNA(Ser) acceptor stem by E-coli seryl-tRNA synthetase
Saks, ME
Sampson, JR
[J]. EMBO JOURNAL, 1996, 15 (11) : 2843 - 2849

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