Presence of chlorogenic acid during in vitro maturation protects porcine oocytes from the negative effects of heat stress

被引：13

作者：

Thanh-Van Nguyen ^{[1
]}

Lanh Thi Kim Do ^{[2
]}

Somfai, Tamas ^{[3
]}

Otoi, Takeshige ^{[4
]}

Taniguchi, Masayasu ^{[5
]}

Kikuchi, Kazuhiro ^{[5
,6
]}

机构：

[1] Natl Inst Anim Sci, Key Lab Anim Cell Technol, Hanoi, Vietnam

[2] Natl Univ Agr, Fac Vet Sci, Hanoi, Vietnam

[3] Natl Agr & Food Res Org NARO, Inst Livestock & Grassland Sci NILGS, Anim Breeding & Reprod Res Div, Tsukuba, Ibaraki, Japan

[4] Tokushima Univ, Fac Biosci & Bioind, Tokushima, Japan

[5] Yamaguchi Univ, Grad Sch Vet Sci, Yamaguchi, Japan

[6] Natl Agr & Food Res Org NARO, Inst Agrobiol Sci NIAS, Div Anim Sci, Tsukuba, Ibaraki, Japan

来源：

ANIMAL SCIENCE JOURNAL | 2019年 / 90卷 / 12期

关键词：

chlorogenic acid; embryo development; heat stress; parthenogenesis; porcine oocyte; DEVELOPMENTAL COMPETENCE; SEASONAL INFERTILITY; SHOCK; APOPTOSIS; DISRUPTION; FLAVONOIDS; NUCLEAR;

D O I：

10.1111/asj.13302

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Chlorogenic acid (CGA) is known to protect oocytes from oxidative stress. Here we investigated the effects of CGA on porcine oocyte maturation under heat stress and subsequent embryonic development after parthenogenetic activation. For in vitro maturation (IVM) at 41.0 degrees C (hyperthermic condition), supplementation of the maturation medium with 50 mu M CGA significantly improved the percentage of matured oocytes and reduced the rate of apoptosis relative to oocytes matured without CGA (p < .05). CGA treatment of oocytes during IVM under hyperthermia tended to increase (p < .1) percentage of blastocyst formation after parthenogenesis and significantly increased (p < .05) the total cell number per blastocyst relative to oocytes matured without CGA. For IVM at 38.5 degrees C (isothermic condition), CGA significantly improved the rate of blastocyst development compared with oocytes matured without CGA (p < .05), but did not affect oocyte maturation, apoptosis rate or the number of cells per embryo. Omission of all antioxidants from the IVM medium significantly reduced the rate of oocyte maturation, but the rate was restored upon addition of CGA. These results demonstrate that CGA is a potent antioxidant that protects porcine oocytes from the negative effects of heat stress, thus reducing the frequency of apoptosis and improving the quality of embryos.

引用

页码：1530 / 1536

页数：7

共 34 条

[1] The effects of oxidative stress on female reproduction: a review [J].

Agarwal, Ashok ;

Aponte-Mellado, Anamar ;

Premkumar, Beena J. ;

Shaman, Amani ;

Gupta, Sajal .

REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 2012, 10

[2] Cathepsin B activity has a crucial role in the developmental competence of bovine cumulus-oocyte complexes exposed to heat shock during in vitro maturation [J].

Balboula, A. Z. ;

Yamanaka, K. ;

Sakatani, M. ;

Kawahara, M. ;

Hegab, A. O. ;

Zaabel, S. M. ;

Takahashi, M. .

REPRODUCTION, 2013, 146 (04) :407-417

[3] Meiotic competence and DNA damage of porcine oocytes exposed to an elevated temperature [J].

Barati, F. ;

Agung, B. ;

Wongsrikeao, P. ;

Taniguchi, A. ;

Nagai, T. ;

Otoi, T. .

THERIOGENOLOGY, 2008, 69 (06) :767-772

[4] Oocyte developmental competence is reduced in sows during the seasonal infertility period [J].

Bertoldo, M. ;

Holyoake, P. K. ;

Evans, G. ;

Grupen, C. G. .

REPRODUCTION FERTILITY AND DEVELOPMENT, 2010, 22 (08) :1222-1229

[5] Promotion of development of bovine embryos produced in vitro by addition of cysteine and β-mercaptoethanol to a chemically defined culture system [J].

Caamano, JN ;

Ryoo, ZY ;

Youngs, CR .

JOURNAL OF DAIRY SCIENCE, 1998, 81 (02) :369-374

[6] Heat stress-induced apoptosis in porcine in vitro fertilized and parthenogenetic preimplantation-stage embryos [J].

Clay Isom, S. ;

Prather, Randall S. ;

Rucker, Edmund B., III .

MOLECULAR REPRODUCTION AND DEVELOPMENT, 2007, 74 (05) :574-581

[7] Effects of Melatonin on the Proliferation and Apoptosis of Sheep Granulosa Cells under Thermal Stress [J].

Fu, Yao ;

He, Chang-Jiu ;

Ji, Peng-Yun ;

Zhuo, Zhi-Yong ;

Tian, Xiu-Zhi ;

Wang, Feng ;

Tan, Dun-Xian ;

Liu, Guo-Shi .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2014, 15 (11) :21090-21104

[8] Microbial metabolism of caffeic acid and its esters chlorogenic and caftaric acids by human faecal microbiota in vitro [J].

Gonthier, M. -P. ;

Remesy, C. ;

Scalbert, A. ;

Cheynier, V. ;

Souquet, J. -M. ;

Poutanen, K. ;

Aura, A. -M. .

BIOMEDICINE & PHARMACOTHERAPY, 2006, 60 (09) :536-540

[9] Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings [J].

Guérin, P ;

El Mouatassim, S ;

Ménézo, Y .

HUMAN REPRODUCTION UPDATE, 2001, 7 (02) :175-189

[10] Low oxygen tension during in vitro maturation of porcine follicular oocytes improves parthenogenetic activation and subsequent development to the blastocyst stage [J].

Iwamoto, M ;

Onishi, A ;

Fuchimoto, D ;

Somfai, T ;

Takeda, K ;

Tagami, T ;

Hanada, H ;

Noguchi, J ;

Kaneko, H ;

Nagai, T ;

Kikuchi, K .

THERIOGENOLOGY, 2005, 63 (05) :1277-1289

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