Application of biochar reduces Ralstonia solanacearum infection via effects on pathogen chemotaxis, swarming motility, and root exudate adsorption

被引：88

作者：

Gu, Yian ^{[1
]}

Hou, Yugang ^{[1
]}

Huang, Dapeng ^{[1
]}

Hao, Zhexia ^{[1
]}

Wang, Xiaofang ^{[1
]}

Wei, Zhong ^{[1
]}

Jousset, Alexandre ^{[1
,2
]}

Tan, Shiyong ^{[3
]}

Xu, Dabing ^{[4
]}

Shen, Qirong ^{[1
]}

Xu, Yangchun ^{[1
]}

Friman, Ville-Petri ^{[5
]}

机构：

[1] Nanjing Agr Univ, Jiangsu Prov Key Lab Organ Solid Waste Utilizat, Natl Engn Res Ctr Organ Based Fertilizers, Weigang 1, Nanjing 210095, Jiangsu, Peoples R China

[2] Univ Utrecht, Inst Environm Biol Ecol & Biodivers, Utrecht, Netherlands

[3] Hunan Taigu Biotechnol Co Ltd, Minist Agr, Key Lab Plant Nutr & Biol Fertilizer, Changsha 410205, Hunan, Peoples R China

[4] Hubei Acad Agr Sci, Plant Protect & Soil Fertilizer Inst, Wuhan 430064, Peoples R China

[5] Univ York, Dept Biol, Wentworth Way, York YO10 5DD, N Yorkshire, England

来源：

PLANT AND SOIL | 2017年 / 415卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

Adsorption; Bacterial wilt; Biochar; Disease control; Root exudate; BACTERIAL WILT; ESCHERICHIA-COLI; ACTIVATED CARBON; PARTICLE-SIZE; SOIL; VIRULENCE; PLANT; WATER; RESISTANCE; AMENDMENTS;

D O I：

10.1007/s11104-016-3159-8

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

We evaluated the efficacy of biochar application for suppressing bacterial wilt of tomato and identified the potential underlying mechanisms involved in the disease control. We measured the impact of two different sized biochar (53-120 mu m and 380-830 mu m) on bacterial wilt incidence in a greenhouse experiment. The efficiency of different sized biochar for the adsorption of tomato root exudates and the pathogen was further examined in vitro. We also quantified the effects of biochar and tomato root exudates on two pathogen virulence factors, chemotaxis, swarming motility and examined the effect of biochar on pathogen root colonization. Fine biochar application (3%; w:w) significantly decreased the bacterial wilt incidence by 19.9%. Biochar with different particle size had similar adsorption capacity for root exudates, while fine biochar was efficient (91%) in pathogen adsorption. Root exudates and fine biochar increased the chemotaxis ability of pathogen, while fine biochar reduced pathogen swarming motility and rhizosphere colonization. Application of fine biochar can significantly decreased bacterial wilt incidence. This was mechanistically explained by biochar ability to 1) adsorb pathogen directly and indirectly via adsorption of root exudates (based on pathogen chemotaxis) and to 2) directly suppress pathogen swarming motility and subsequent root colonization.

引用

页码：269 / 281

页数：13

共 66 条

[1] Influence of Feedstock and Pyrolysis Temperature of Biochar Amendments on Transport of Escherichia coli in Saturated and Unsaturated Soil [J].