MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines

被引：67

作者：

Hummel, Richard ^{[1
,2
]}

Sie, Corina ^{[1
]}

Watson, David I. ^{[1
]}

Wang, Tingting ^{[1
]}

Ansar, Alfiya ^{[1
]}

Michael, Michael Z. ^{[3
]}

Van der Hoek, Mark ^{[4
]}

Haier, Joerg ^{[5
]}

Hussey, Damian J. ^{[1
]}

机构：

[1] Flinders Univ S Australia, Med Ctr, Dept Surg, Bedford Pk, SA 5042, Australia

[2] Univ Munster, Dept Gen & Visceral Surg, D-48149 Munster, Germany

[3] Flinders Univ S Australia, Dept Gastroenterol & Hepatol, Bedford Pk, SA 5042, Australia

[4] Adelaide Microarray Ctr, SA Pathol, Adelaide, SA 5000, Australia

[5] Univ Munster, Ctr Comprehens Canc, D-48149 Munster, Germany

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2014年 / 20卷 / 40期

基金：

英国医学研究理事会;

关键词：

Esophageal cancer; MicroRNA; Chemotherapy; Resistance; Target; MULTIDRUG-RESISTANCE; CONFERS RESISTANCE; CARCINOMA; CHEMORADIOTHERAPY; METAANALYSIS; SURVIVAL; RECEPTOR;

D O I：

10.3748/wjg.v20.i40.14904

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: To investigate expression of microRNA (miRNA) and potential targets in chemotherapy resistant esophageal cancer cell lines. METHODS: An in-vitro model of acquired chemotherapy resistance in esophageal adeno- (EAC) and squamous cell carcinoma (ESCC) cells was used, and microRNA expression profiles for cisplatin or 5-fluorouracil (5-FU) resistant variants vs chemotherapy sensitive controls were compared using microarray and quantitative real-time polymerase chain reaction (PCR). The expression of chemotherapy-relevant genes potentially targeted by the dysregulated microRNAs in the chemotherapy resistant variants was also evaluated. RESULTS: Chemotherapy resistant sublines were found to have specific miRNA signatures, and these miRNA signatures were different for the cisplatin vs 5-FU resistant cells from the same tumor cell line, and also for EAC vs ESCC cells with resistance to the same specific chemotherapy agent. Amongst others, miR-27b-3p, miR-193b-3p, miR-192-5p, miR-378 a-3p, miR-125a-5p and miR-18a-3p were dysregulated, consistent with negative posttranscriptional control of KRAS, TYMS, ABCC3, CBL-B and ERBB2 expression via these miRNAs. CONCLUSION: The current study supports the hypothesis that microRNA expression has an impact on chemotherapy resistance in esophageal cancer. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.

引用

页码：14904 / 14912

页数：9

共 28 条

[1] CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].