A Closed Concept of Extractive Whole Cell Microbial Transformation of Benzaldehyde into l-Phenylacetylcarbinol by Saccharomyces cerevisiae in Novel Polyethylene-Glycol-Induced Cloud-Point System

被引：10
|
作者：
Wang, Zhilong [1 ]
Liang, Rui [1 ]
Xu, Jian-He [2 ]
Liu, Yubo [3 ]
Qi, Hanshi [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China
[2] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biocatalysis & Bioproc, Shanghai 200237, Peoples R China
[3] Hunan Microbiol Inst, Changsha 410009, Hunan, Peoples R China
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2010年 / 160卷 / 06期
基金：
中国国家自然科学基金;
关键词：
Microbial transformation; Nonionic surfactant; Extraction; Cloud-point system; Microemulsion; 2-PHASE PARTITIONING BIOREACTORS; AQUEOUS; 2-PHASE; ORGANIC-COMPOUNDS; BACK-EXTRACTION; SURFACTANT; BIOTRANSFORMATION; FERMENTATION; SEPARATION; PHASE; (R)-PHENYLACETYLCARBINOL;
D O I：
10.1007/s12010-009-8695-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Extractive microbial transformation of benzaldehyde into l-phenylacetylcarbinol (l-PAC) by Saccharomyces cerevisiae (Baker's yeast) has been carried out in a novel polyethylene-glycol-induced cloud-point system (PEG-CPS). The extractive microbial transformation in the PEG-CPS and a downstream process for stripping of the product from the microbial transformation broth with microemulsion extraction are demonstrated. The results indicate that the PEG-CPS maintains the advantage of CPS for in situ extraction of polar product in the microbial transformation. At the same time, the utilization of hydrophilic nonionic surfactant in the PEG-CPS is favorable for stripping of product from the nonionic surfactant in the microbial transformation broth by Winsor I microemulsion extraction. Thus, a closed concept of in situ extraction of polar product in microbial transformation and its downstream process of product recovery are fulfilled at the same time.
引用
收藏
页码：1865 / 1877
页数：13
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