MICROBIAL COMMUNITY FUNCTIONAL DIVERSITY IN AN ORGANOCHLORINE CONTAMINATED SITE AND EVALUATION OF BIODEGRADATION EFFICIENCY

A typical chlorinated hydrocarbon contaminated site was selected as the research object in southern China First, Gas Chromatography-Mass Spectrometer (GC-MS) was used to analyze the composition and concentration of pollutants in soil and groundwater samples. Then, we investigated the microbial community structure, composition, and abundance by high-throughput sequencing analysis. Principal component analysis and environmental factor correlation analysis were further used to illustrate the functional diversity of microbial communities. Eventually, the results showed that the composition and structure of the microbial community were significantly different due to the various degrees of pollution in the soil and groundwater. In the soil with high pollution degree, the functional diversity of the microbial community was notably different from that of non-polluted or slightly polluted soil. This indicated that soil microbial physiological activities and community functional diversity could be affected by the residual concentration of chlorinated hydrocarbons in contaminated soils. The dominant microbial community in the soil was Penicillium, which had a higher abundance in the soil environment, while the Phylum Firmicutes were more sensitive species. In addition, as stain-resistant bacteria, Brevundimonas, Rhizobium Frank, and Pseudomonas were observed in the groundwater, where Lasiodiplodia was the dominant microbial community, while Fusarium was more sensitive species.