Semi-automated simple sequence repeat analysis reveals narrow genetic base in Indian potato cultivars

被引：13

作者：

Chimote, VP ^{[1
]}

Chakrabarti, SK ^{[1
]}

Pattanayak, D ^{[1
]}

Naik, PS ^{[1
]}

机构：

[1] Cent Potato Res Inst, Div Crop Improvement, Shimla 171001, Himachal Prades, India

来源：

BIOLOGIA PLANTARUM | 2004年 / 48卷 / 04期

关键词：

genetic relationship; microsatellites; simple sequence repeats polymorphism; Solanum tuberosum;

D O I：

10.1023/B:BIOP.0000047146.69936.ed

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A study was conducted to generate fingerprints of thirty-two Indian potato cultivars using capillary electrophoresis based semi-automated simple sequence repeat analysis. Five fluorescent-tagged primer pairs (STPOACUTR, LEGAST1, STPOAC58, STM0030 and STM0031) used in this study yielded 43 alleles at 16 different loci, showing multi-loci resolving character. The estimated similarity between the cultivars was in the range of 0.72 to 0.98 indicating narrow genetic relationship. None of the primer set alone could differentiate all 32 cultivars included in this study. However, two primer sets STM-0031 and STPOAC58 amplifying 12 and 9 polymorphic alleles, respectively, could together distinguish all of them. The results indicated usefulness of semi-automated capillary electrophoresis in quick and reproducible SSR genotyping of potato cultivars.

引用

页码：517 / 522

页数：6

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