A subdomain of the endoplasmic reticulum forms a cradle for autophagosome formation

被引：822

作者：

Hayashi-Nishino, Mitsuko ^{[2
,3
]}

Fujita, Naonobu ^{[1
]}

Noda, Takeshi ^{[1
]}

Yamaguchi, Akihito ^{[2
]}

Yoshimori, Tamotsu ^{[1
]}

Yamamoto, Akitsugu ^{[3
]}

机构：

[1] Osaka Univ, Dept Cellular Regulat, Microbial Dis Res Inst, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Dept Cell Membrane Biol, Inst Sci & Ind Res, Osaka 5670047, Japan

[3] Nagahama Inst Biosci & Technol, Fac Biosci, Shiga 5260829, Japan

来源：

NATURE CELL BIOLOGY | 2009年 / 11卷 / 12期

关键词：

RAT HEPATOCYTES; ISOLATION MEMBRANES; PROTEIN; DEGRADATION; TOMOGRAPHY; VACUOLES; CELLS;

D O I：

10.1038/ncb1991

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Autophagy is a bulk degradation process in eukaryotic cells and has fundamental roles in cellular homeostasis. The origin and source of autophagosomal membranes are long-standing questions in the field. Using electron microscopy, we show that, in mammalian culture cells, the endoplasmic reticulum ( ER) associates with early autophagic structures called isolation membranes (IMs). Overexpression of an Atg4B mutant, which causes defects in autophagosome formation, induces the accumulation of ER-IM complexes. Electron tomography revealed that the ER-IM complex appears as a subdomain of the ER that formed a cradle encircling the IM, and showed that both ER and isolation membranes are interconnected.

引用

页码：1433 / U102

页数：11

共 22 条

[1] Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum [J].