Separation of gallic acid from Cornus officinalis Sieb. et Zucc by high-speed counter-current chromatography

被引：47

作者：

Tian, GL ^{[1
]}

Zhang, TY

Yang, FQ

Ito, Y

机构：

[1] Beijing Inst New Technol Applicat, Beijing 100035, Peoples R China

[2] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 886卷 / 1-2期

关键词：

Cornus officinalis; counter-current chromatography; gallic acid;

D O I：

10.1016/S0021-9673(00)00480-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Gallic acid was separated from a n-butanol extract of the fruit of Cornus officinalis Sieb. et Zucc by high-speed countercurrent chromatography in two steps using two solvent systems composed of ethyl-acetate-ethanol n-butanol-water (5:1.8:6, v/v/v) and ethyl acetate-ethanol-water (5:0.5:6, v/v/v) successively. From 1 g of n-butanol extract the method produced 60 mg of gallic acid at a purity of 97%. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：309 / 312

页数：4