Analysis of epitope recognition of antibodies induced by DNA immunization against hemagglutinin protein of influenza A virus

被引：25
作者：
Tonegawa, K
Nobusawa, E
Nakajima, K
Takashi, K
Kutsuna, T
Kuroda, K
Shibata, T
Harada, Y
Nakamura, A
Itoh, M
机构：
[1] Nagoya City Univ, Grad Sch Med Sci, Dept Virol, Mizuho Ku, Nagoya, Aichi 4678601, Japan
[2] Nagoya City Univ, Grad Sch Med Sci, Dept Int Med & Bioregulat, Mizuho Ku, Nagoya, Aichi 4678601, Japan
[3] Nihon Univ, Dept Immunol & Microbiol, Sch Med, Itabashi Ku, Tokyo 1738601, Japan
[4] Osaka Univ Pharmaceut Sci, Dept Virol & Immunol, Takatsuki, Osaka 5691094, Japan
来源：
VACCINE | 2003年 / 21卷 / 23期
关键词：
influenza virus; hemagglutinin; DNA immunization;
D O I：
10.1016/S0264-410X(03)00257-3
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
In an effort to find efficient DNA vaccine candidates, cDNA of influenza A virus hemagglutinin (HA) gene and several derived mutants were injected into mice using a gene gun. Mice immunized with HA1 DNA, with or without a membrane domain, showed a humoral immune response and the survival rate against homologous virus challenge was comparable to that of mice injected with HA DNA. In order to analyze epitopes recognized by antibodies induced by gene gun immunization, we used a binding assay employing the chimeric HA protein method. Serum antibodies of mice immunized with HA DNA recognized the HA1 domain but not the HA2 domain. In addition, antisera obtained from mice immunized with HA1 DNA reacted with each of the known antigenic sites on the HA1 domain, similar to the results obtained with HA DNA immunization. (C) 2003 Published by Elsevier Science Ltd.
引用
收藏
页码：3118 / 3125
页数：8
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