Regulation of the transcription factor, CTCF, by phosphorylation with protein kinase CK2

被引：55
|
作者：
El-Kady, A
Klenova, E
机构：
[1] Univ Essex, Dept Biol Sci, Colchester CO4 3SQ, Essex, England
[2] Univ Oxford, Dept Biochem, Genet Unit, Oxford OX1 3QU, England
来源：
FEBS LETTERS | 2005年 / 579卷 / 06期
关键词：
CTCF; protein kinase CK2; c-myc; phosphorylation; transcription;
D O I：
10.1016/j.febslet.2005.01.044
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
CTCF is a transcription factor involved in various aspects of gene regulation. We previously reported that CTCF function is modulated by protein kinase CK2. In this report we investigate further the role of CK2 in regulating the transcriptional properties of CTCF. We demonstrate that coexpression of CTCF with CK2 switches function of CTCF from repressor to activator. The non-phosphorylatable mutant increases repression by CTCF and potentiates the growth-suppressive ability of the protein, whereas the phospho-mimetic mutant behaves in the opposite fashion. Mutation of the individual serines reveals that Serine 612 is a critical residue in regulation of CTCF by CK2. (C) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：1424 / 1434
页数：11
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