Antibacterial Activity and Cytotoxicity of Silver Chloride/Silver Nanocomposite Synthesized by a Bacterium Isolated from Antarctic Soil

被引:9
作者
Rolim, Wallace R. [1 ,2 ]
Lamilla, Claudio [3 ,4 ]
Pieretti, Joana C. [1 ,2 ]
Nascimento, Monica H. M. [1 ,2 ]
Ferreira, Fabio F. [1 ,2 ]
Tortella, Gonzalo R. [4 ,5 ]
Diez, Maria C. [4 ,5 ]
Barrientos, Leticia [5 ,6 ]
Rubilar, Olga [4 ,5 ]
Seabra, Amedea B. [1 ,2 ]
机构
[1] Fed Univ ABC UFABC, Ctr Nat & Human Sci CCNH, Av Estados 5001, BR-09210580 Santo Andre, SP, Brazil
[2] Fed Univ ABC UFABC, Nanomed Res Unit NANOMED, Santo Andre, SP, Brazil
[3] Fed Univ ABC UFABC, Ctr Engn Modeling & Appl Social Sci, Santo Andre, SP, Brazil
[4] Univ La Frontera, Dept Chem Engn, Temuco, Chile
[5] Univ La Frontera, Biotechnol Res Ctr Appl Environm CIBAMA BIOREN, Temuco, Chile
[6] Univ La Frontera, Lab Appl Mol Biol, Ave Alemania 0458, Temuco, Chile
关键词
Arthrobacter sp; Biogenic synthesis; Cytotoxicity; Silver chloride nanoparticles; Silver nanoparticles; Antibacterial activity; EXTRACT-MEDIATED SYNTHESIS; RAY-POWDER DIFFRACTOMETER; AG-AT-AGCL; EXTRACELLULAR SYNTHESIS; ANTIMICROBIAL ACTIVITY; GREEN SYNTHESIS; CULTURE SUPERNATANT; BIOGENIC SYNTHESIS; OXIDATIVE STRESS; LOW-TEMPERATURE;
D O I
10.1007/s12668-019-00693-1
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
This study aims to describe the antibacterial and cytotoxic properties of a nanocomposite composed of silver chloride and silver nanoparticles (AgCl/Ag) biogenically synthesized using Arthrobacter sp., a non-pathogenic Antarctic soil bacterium. Spherical nanoparticles (hydrodynamic size of 42 nm) coated with biomolecules derived from Arthrobacter sp. were obtained. The antibacterial activity of the nanocomposite was demonstrated against Gram-positive and Gram-negative bacterial strains, including the multidrug-resistant Pseudomonas aeruginosa KPC 37. Furthermore, the cytotoxicity of AgCl/Ag nanocomposite was evaluated against the non-tumoral (Vero) and tumoral (SW-1353) cell lines. The nanocomposite was not cytotoxic to the Vero cells at concentrations below 150 mu g/mL, whereas at concentrations above 25 mu g/mL, a significant cytotoxic effect was observed for the SW-1353 cells. Interestingly, at 150 mu g/mL, the nanocomposite demonstrated a potent antibacterial activity for all bacterial strains evaluated. To the best of our knowledge, this is the first report describing the green synthesis of AgCl/Ag nanocomposite by the bacterium Arthrobacter sp. isolated from Antarctic soil. The obtained nanocomposite may find important biomedical applications, with potent antibacterial and antitumoral effects and low toxicity to healthy cells.
引用
收藏
页码:136 / 148
页数:13
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