Design of clone-specific probes from genome sequences for rapid PCR-typing of outbreak pathogens

被引：15

作者：

Lopez-Camacho, E. ^{[1
]}

Rentero, Z. ^{[1
]}

Ruiz-Carrascoso, G. ^{[1
]}

Wesselink, J-J ^{[2
]}

Perez-Vazquez, M. ^{[3
]}

Lusa-Bernal, S. ^{[2
]}

Gomez-Puertas, P. ^{[4
]}

Kingsley, R. A. ^{[5
]}

Gomez-Sanchez, P. ^{[1
]}

Campos, J. ^{[3
]}

Oteo, J. ^{[3
]}

Mingorance, J. ^{[1
]}

机构：

[1] Hosp Univ La Paz, Microbiol Serv, IdiPaz, Madrid 28046, Spain

[2] Biomol Informat SL, Madrid, Spain

[3] Inst Salud Carlos III, Ctr Nacl Microbiol, Dept Bacteriol, Antibiot Lab, Madrid, Spain

[4] Severo Ochoa CSIC UAM, Ctr Biol Mol, Madrid, Spain

[5] Wellcome Trust Sanger Inst, Cambridge, England

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2014年 / 20卷 / 11期

关键词：

Bacterial genomes; hospital infection; Klebsiella pneumoniae; OXA-48; PCR typing; OXA-48-PRODUCING KLEBSIELLA-PNEUMONIAE; DIVERSILAB SYSTEM; SPAIN; ENTEROBACTERIACEAE; INFECTIONS;

D O I：

10.1111/1469-0691.12616

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The genome sequence of one OXA-48-producing Klebsiella pneumoniae belonging to sequence type (ST) 405, and three belonging to ST11, were used to design and test ST-specific PCR assays for typing OXA-48-producing K.pneumoniae. The approach proved to be useful for in-house development of rapid PCR typing assays for local outbreak surveillance.

引用

页码：O891 / O893

页数：3

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