Antiviral Lipopeptide-Cell Membrane Interaction Is Influenced by PEG Linker Length

被引：14

作者：

Augusto, Marcelo T. ^{[1
]}

Hollmann, Axel ^{[1
,2
,3
]}

Porotto, Matteo ^{[4
,5
]}

Moscona, Anne ^{[4
,5
,6
,7
]}

Santos, Nuno C. ^{[1
]}

机构：

[1] Univ Lisbon, Fac Med, Inst Med Mol, Av Prof Egas Moniz, P-1649028 Lisbon, Portugal

[2] Natl Univ Quilmes, Inst Basic & Appl Microbiol, Lab Mol Microbiol, Roque Saenz Pena 352, RA-1876 Bernal, Buenos Aires, Argentina

[3] Natl Univ Santiago del Estero, CONICET, CITSE, Lab Biointerfaces & Biomimet Syst, RA-4200 Santiago Del Estero, Argentina

[4] Columbia Univ, Med Ctr, Ctr Host Pathogen Interact, 701 W 168th, New York, NY 10032 USA

[5] Columbia Univ, Med Ctr, Dept Pediat, 701 W 168th, New York, NY 10032 USA

[6] Columbia Univ, Med Ctr, Dept Microbiol & Immunol, 701 W 168th, New York, NY 10032 USA

[7] Columbia Univ, Med Ctr, Dept Physiol & Cellular Biophys, 701 W 168th, New York, NY 10032 USA

来源：

MOLECULES | 2017年 / 22卷 / 07期

关键词：

paramyxoviruses; peptides; antiviral; cholesterol; membranes; FUSION INHIBITOR PEPTIDES; LIPID RAFT MICRODOMAINS; MEASLES-VIRUS INFECTION; BROAD-SPECTRUM; VIRAL FUSION; NIPAH VIRUS; ENVELOPED VIRUSES; ENTRY INHIBITORS; CHOLESTEROL; HIV-1;

D O I：

10.3390/molecules22071190

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A set of lipopeptides was recently reported for their broad-spectrum antiviral activity against viruses belonging to the Paramyxoviridae family, including human parainfluenza virus type 3 and Nipah virus. Among them, the peptide with a 24-unit PEG linker connecting it to a cholesterol moiety (VG-PEG24-Chol) was found to be the best membrane fusion inhibitory peptide. Here, we evaluated the interaction of the same set of peptides with biomembrane model systems and isolated human peripheral blood mononuclear cells (PBMC). VG-PEG24-Chol showed the highest insertion rate and it was among the peptides that induced a larger change on the surface pressure of cholesterol rich membranes. This peptide also displayed a high affinity towards PBMC membranes. These data provide new information about the dynamics of peptide-membrane interactions of a specific group of antiviral peptides, known for their potential as multipotent paramyxovirus antivirals.

引用

页数：10

共 47 条

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