New studies on gum ghatti (Anogeissus latifolia) part 5: The conformational properties of gum ghatti

被引：15

作者：

Kang, Ji ^{[1
]}

Guo, Qingbin ^{[1
]}

Wang, Qi ^{[1
]}

Phillips, Glyn O. ^{[2
,3
]}

Cui, Steve W. ^{[1
]}

机构：

[1] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada

[2] Glyndwr Univ, Glyn O Phillips Hydrocolloid Res Ctr, Wrexham LL11 2AW, Wales

[3] Phillips Hydrocolloids Res Ltd, London W1S 4AQ, England

来源：

FOOD HYDROCOLLOIDS | 2015年 / 43卷

关键词：

Gum ghatti; Conformation; Random coil; Hydrocolloid emulsifier; Light scattering; SIZE-EXCLUSION CHROMATOGRAPHY; STATIC LIGHT-SCATTERING; MOLECULAR-WEIGHT;

D O I：

10.1016/j.foodhyd.2014.04.042

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The conformational properties of gum ghatti and its fractions were investigated using high performance size exclusion chromatography (HPSEC) coupled with multiple detectors, static light scattering (SLS) and dynamic light scattering (DLS) methods. The measured Mark-Houwink equation parameters alpha (0.5 < alpha < 0.8) indicated a random coil conformation of four fractions: F50 was more rigid than the other fractions, whereas F65 exhibited a loosely extended chain that is close to a spherical conformation. DLS and SLS measurements in aggregate free (0.5 M NaOH) solution further confirmed the conformational properties. The high positive A(2) value of each fraction indicated that 0.5 M NaOH solution is a good solvent for gum ghatti. The values of R-g and R-h decreased with molecular weight in the order of F80, F65 and F50. The less branched fraction F50 (rho = 1.694) has a random coil conformation in 0.5 M NaOH solution, whereas F65 and F80 exhibited random coil close to spherical conformation due to their highly branched structure. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：25 / 30

页数：6

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