Differential effects of sevoflurane on the metastatic potential and chemosensitivity of non-small-cell lung adenocarcinoma and renal cell carcinoma in vitro

被引：80
作者：
Ciechanowicz, S. [1 ]
Zhao, H. [1 ]
Chen, Q. [1 ,2 ]
Cui, J. [1 ]
Mi, E. [1 ]
Mi, E. [1 ]
Lian, Q. [3 ]
Ma, D. [1 ]
机构：
[1] Imperial Coll London, Chelsea & Westminster Hosp, Fac Med, Dept Surg & Canc Anaesthet Pain Med & Intens Care, London, England
[2] Third Mil Med Univ, Southwest Hosp, Dept Anesthesiol, Chongqing, Peoples R China
[3] Wenzhou Med Univ, Affiliated Hosp 2, Wenzhou, Peoples R China
来源：
BRITISH JOURNAL OF ANAESTHESIA | 2018年 / 120卷 / 02期
关键词：
anaesthetic drugs; carcinoma; kidney; lung; metastasis; SIGNALING PATHWAY; CANCER CELLS; GROWTH; EXPRESSION; APOPTOSIS; INVASION; INJURY;
D O I：
10.1016/j.bja.2017.11.066
中图分类号：
R614 [麻醉学];
学科分类号：
100217 ;
摘要：
Background: Increasing evidence suggests that perioperative factors including anaesthetics influence cancer recurrence and metastasis after surgery. This study investigated the influence of sevoflurane on the response of lung and renal cancer cells to cisplatin, with focus on transforming growth factor-beta (TGF-beta) and osteopontin (OPN) that are both closely associated with cancer tumorigenesis and metastasis. Methods: Non-small cell lung adenocarcinoma (A549) and renal cell carcinoma (RCC4) cells were exposed to 3.6% sevoflurane for two hrs. Malignant potential represented by cell viability, migration, chemosensitivity to cisplatin was evaluated. Expression of OPN, TGF-beta 1, TGF-beta receptor type II (TGF-beta RII) and the canonical downstream effector Smad3 was assessed. SiRNA knockdown of TGF-beta 1 and OPN and chemical inhibition of TGF-beta RI/II was performed. Results: Sevoflurane reduced cell viability (0.394) versus control (0.459) (P < 0.01), enhanced chemosensitivity but had no effect on migration of A549 cells. It enhanced viability (0.467) versus control (0.347) (P < 0.001), chemoresistance and migration of RCC4. In A549, there was enhanced nuclear Smad3. In RCC4, TGF-beta RII and OPN were upregulated, while TGF-beta 1 was over-expressed with reduced nuclear Smad3. TGF-beta RII inhibition and OPN knockdown abolished sevoflurane-mediated viability, and migration, respectively, in RCC4. Conclusions: Sevoflurane promotes the metastatic potential of renal carcinoma, but not of non-small cell lung cancer. This may be associated with its differential effect on cellular signalling including TGF-beta. Our findings indicate that sevoflurane may have different effects on the metastatic potential and chemosensitivity of different tumour types.
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页码：368 / 375
页数：8
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