SARS-CoV-2 variants B.1.351 and P.1 escape from neutralizing antibodies

被引：707

作者：

Hoffmann, Markus ^{[1
,2
]}

Arora, Prerna ^{[1
,2
]}

Gross, Rudiger ^{[3
]}

Seidel, Alina ^{[3
]}

Hornich, Bojan F. ^{[4
]}

Hahn, Alexander S. ^{[4
]}

Kruger, Nadine ^{[1
]}

Graichen, Luise ^{[1
]}

Hofmann-Winkler, Heike ^{[1
]}

Kempf, Amy ^{[1
,2
]}

Winkler, Martin S. ^{[5
]}

Schulz, Sebastian ^{[6
]}

Jack, Hans-Martin ^{[6
]}

Jahrsdorfer, Bernd ^{[7
,8
,9
]}

Schrezenmeier, Hubert ^{[7
,8
,9
]}

Muller, Martin ^{[10
]}

Kleger, Alexander ^{[10
]}

Munch, Jan ^{[3
,11
]}

Pohlmann, Stefan ^{[1
,2
]}

机构：

[1] German Primate Ctr, Infect Biol Unit, Kellnerweg 4, D-37077 Gottingen, Germany

[2] Georg August Univ Gottingen, Facu Biol & Psychol, Wilhelmspl 1, D-37073 Gottingen, Germany

[3] Ulm Univ, Med Ctr, Inst Mol Virol, Meyerhofstr 1, D-89081 Ulm, Germany

[4] German Primate Ctr, Infect Biol Unit, Jr Res Grp Herpesviruses, Kellnerweg 4, D-37077 Gottingen, Germany

[5] Georg August Univ Gottingen, Univ Gottingen, Med Ctr, Dept Anaesthesiol, Robert Koch Str 40, D-37075 Gottingen, Germany

[6] Friedrich Alexander Univ Erlangen Nurnberg, Dept Internal Med 3, Div Mol Immunol, Gluckstr 6, D-91054 Erlangen, Germany

[7] Ulm Univ, Dept Transfus Med, Helmholtzstr 10, D-89081 Ulm, Germany

[8] German Red Cross Blood Transfus Serv Baden Wurtte, Inst Clin Transfus Med & Immunogenet, Helmholtzstr 10, D-89081 Ulm, Germany

[9] Univ Hosp Ulm, Helmholtzstr 10, D-89081 Ulm, Germany

[10] Ulm Univ Hosp, Dept Internal Med 1, Albert Einstein Allee 23, D-89081 Ulm, Germany

[11] Ulm Univ, Med Ctr, Core Facil Funct Peptid, Meyerhofstr 4, D-89081 Ulm, Germany

来源：

CELL | 2021年 / 184卷 / 09期

关键词：

SPIKE PROTEIN; INFECTION; TMPRSS2; VIRUS; ACE2;

D O I：

10.1016/j.cell.2021.03.036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The global spread of SARS-CoV-2/COVID-19 is devastating health systems and economies worldwide. Recombinant or vaccine-induced neutralizing antibodies are used to combat the COVID-19 pandemic. However, the recently emerged SARS-CoV-2 variants B.1.1.7 (UK), B.1.351 (South Africa), and P.1 (Brazil) harbor mutations in the viral spike (S) protein that may alter virus-host cell interactions and confer resistance to inhibitors and antibodies. Here, using pseudoparticles, we show that entry of all variants into human cells is susceptible to blockade by the entry inhibitors soluble ACE2, Camostat, EK-1, and EK-1-C4. In contrast, entry of the B.1.351 and P.1 variant was partially (Casirivimab) or fully (Bamlanivimab) resistant to antibodies used for COVID-19 treatment. Moreover, entry of these variants was less efficiently inhibited by plasma from convalescent COVID-19 patients and sera from BNT162b2-vaccinated individuals. These results suggest that SARS-CoV-2 may escape neutralizing antibody responses, which has important implications for efforts to contain the pandemic.

引用

页码：2384 / +

页数：22

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