Protective effects of black currant (Ribes nigrum L.) extract on hydrogen peroxide-induced damage in lung fibroblast MRC-5 cells in relation to the antioxidant activity

被引：32

作者：

Jia, Na ^{[1
,2
]}

Li, Tiejing ^{[2
]}

Diao, Xinping ^{[3
]}

Kong, Baohua ^{[2
]}

机构：

[1] Bohai Univ, Food Sci Res Inst, Food Safety Key Lab Liaoning Prov, Jinzhou 121013, Liaoning, Peoples R China

[2] Northeast Agr Univ, Coll Food Sci, Harbin 150030, Heilongjiang, Peoples R China

[3] Northeast Agr Univ, Coll Anim Sci, Harbin 150030, Heilongjiang, Peoples R China

来源：

JOURNAL OF FUNCTIONAL FOODS | 2014年 / 11卷

基金：

中国国家自然科学基金;

关键词：

Black currant extract; Antioxidant activity; Protective effects; Hydrogen peroxide-induced; damage; MRC-5; cells; RADICAL SCAVENGING ACTIVITY; OXIDATIVE STRESS; PHENOLIC ANTIOXIDANTS; RED CURRANTS; CANCER-CELLS; PC12; CELLS; IN-VITRO; ANTHOCYANINS; APOPTOSIS; POLYPHENOLS;

D O I：

10.1016/j.jff.2014.09.011

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A black currant (Ribes nigrum L.) extract (BCE) was prepared to investigate its protective effects against damage to lung fibroblast MRC-5 cells induced by H2O2. BCE exhibited strong scavenging activities against hydroxyl (center dot OH) and superoxide anion radicals (O-2(center dot-)) in vitro. Exposing MRC-5 cells to 11202 reduced the cell viability; the activities of antioxidant enzymes, including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), and cell apoptosis while increasing the thiobarbituric acid reactive substances (TBARS) content. Pretreating cells with different concentrations of BCE (0, 2.5, 5 and 10 mg/ml) enhanced the cell viability and antioxidant enzyme activities while decreasing the TBARS content. Morphological observations and flow cytometric analysis revealed that BCE alleviated the cell apoptosis induced by H2O2. These protective effects of BCE on MRC-5 cells suggest that black currants may possess the ability to eliminate over-produced ROS in humans. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：142 / 151

页数：10

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