Electrochemical studies of CuTMAP interaction with DNA and determination of DNA

被引：27
作者：
Qu, F [1 ]
Li, NQ [1 ]
Jiang, YY [1 ]
机构：
[1] Peking Univ, Dept Chem, Beijing 100871, Peoples R China
来源：
MICROCHEMICAL JOURNAL | 1998年 / 58卷 / 01期
基金：
中国国家自然科学基金;
关键词：
voltammetry; metalloporphyrin CuTMAP; DNA;
D O I：
10.1006/mchj.1997.1511
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Electrochemical investigation of CuTMAP and its interaction with DNA at a Hg electrode is reported for the first time. The reduction of CuTMAP under our experimental conditions involves reduction of 2e of Cu2+ and 2e of TMAP. Reaction of DNA with CuTMAP forms an electrochemically nonactive complex, which results in a decrease in the CuTMAP equilibrium concentration and its reduction current. The decrease in peak current is proportional to DNA concentration and can be used to estimate DNA concentration. (C) 1998 Academic Press.
引用
收藏
页码：39 / 51
页数：13
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