TRIM59 facilitates the proliferation of colorectal cancer and promotes metastasis via the PI3K/AKT pathway

被引：68
作者：
Sun, Ye [1 ]
Ji, Bing [1 ]
Feng, Yifei [1 ]
Zhang, Yue [1 ]
Ji, Dongjian [1 ]
Zhu, Chunyan [1 ]
Wang, Sen [1 ]
Zhang, Chuan [1 ]
Zhang, Dongsheng [1 ]
Sun, Yueming [1 ]
机构：
[1] Nanjing Med Univ, Affiliated Hosp 1, Dept Colorectal Surg, 300 Guangzhou Rd, Nanjing 210029, Jiangsu, Peoples R China
来源：
ONCOLOGY REPORTS | 2017年 / 38卷 / 01期
关键词：
TRIM59; colorectal cancer; EMT; PI3K/AKT pathway; biomarker; CELL-PROLIFERATION; TRIPARTITE MOTIF; FAMILY PROTEINS; MIGRATION; OSTEOSARCOMA; KNOCKDOWN; APOPTOSIS; SURVIVAL; SNAIL;
D O I：
10.3892/or.2017.5654
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Tripartite motif-containing 59 (TRIM59) belongs to the tripartite motif (TRIM) protein family and is upregulated in various malignancies. However, its expression in colorectal cancer (CRC) is still unknown. In the present study, we examined the expression and biological function of TRIM59 in CRC. We analyzed CRC tissues and cells by quantitative real-time polymerase chain reaction. Kaplan-Meier survival analysis was used to evaluate the prognostic significance of TRIM59 in CRC patients. Furthermore, we investigated the role of TRIM59 in CRC growth and metastasis. The potential mechanism underlying the regulation of cell metastasis by TRIM59 was determined by western blotting. TRIM59 expression was conspicuously overexpressed in CRC tissues and CRC cell lines compared to that noted in the corresponding normal control cells. Patients with higher TRIM59 expression had poorer prognosis. Furthermore, knockdown of TRIM59 suppressed cell proliferation through the induction of apoptosis and inhibited migration and invasion significantly in vitro. Further investigation revealed that knockdown of TRIM59 effectively reversed the expression of epithelial-mesenchymal transformation-related proteins vimentin, Snail and E-cadherin. Our preliminary results confirm that TRIM59 can be mediated by PI3K/AKT signaling. TRIM59 functions as an oncogene in CRC progression, which could be a novel target for the detection and treatment of CRC.
引用
收藏
页码：43 / 52
页数：10
相关论文
共 27 条
[1]   Knockdown of Tripartite-59 (TRIM59) Inhibits Cellular Proliferation and Migration in Human Cervical Cancer Cells [J].
Aierken, Gulijiahan ;
Seyiti, Ayinuer ;
Alifu, Mayinuer ;
Kuerban, Gulina .
ONCOLOGY RESEARCH, 2017, 25 (03) :381-388
[2]   STC2 promotes the epithelial-mesenchymal transition of colorectal cancer cells through AKT-ERK signaling pathways [J].
Chen, Bing ;
Zeng, Xiao ;
He, Yu ;
Wang, Xixi ;
Liang, Ziwei ;
Liu, Jingjing ;
Zhang, Peng ;
Zhu, Hongxia ;
Xu, Ningzhi ;
Liang, Shufang .
ONCOTARGET, 2016, 7 (44) :71400-71416
[3]   Cancer Statistics in China, 2015 [J].
Chen, Wanqing ;
Zheng, Rongshou ;
Baade, Peter D. ;
Zhang, Siwei ;
Zeng, Hongmei ;
Bray, Freddie ;
Jemal, Ahmedin ;
Yu, Xue Qin ;
He, Jie .
CA-A CANCER JOURNAL FOR CLINICIANS, 2016, 66 (02) :115-132
[4]   TRIM66 overexpresssion contributes to osteosarcoma carcinogenesis and indicates poor survival outcome [J].
Chen, Yu ;
Guo, Yongfei ;
Yang, Haisong ;
Shi, Guodong ;
Xu, Guohua ;
Shi, Jiangang ;
Yin, Na ;
Chen, Deyu .
ONCOTARGET, 2015, 6 (27) :23708-23719
[5]   TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity [J].
Gack, Michaela U. ;
Shin, Young C. ;
Joo, Chul-Hyun ;
Urano, Tomohiko ;
Liang, Chengyu ;
Sun, Lijun ;
Takeuchi, Osamu ;
Akira, Shizuo ;
Chen, Zhijian ;
Inoue, Satoshi ;
Jung, Jae U. .
NATURE, 2007, 446 (7138) :916-U2
[6]  
Glockzin Gabriel, 2016, World J Gastrointest Pharmacol Ther, V7, P343, DOI 10.4292/wjgpt.v7.i3.343
[7]   Structural basis for PRYSPRY-mediated tripartite motif (TRIM) protein function [J].
James, Leo C. ;
Keeble, Anthony H. ;
Khan, Zahra ;
Rhodes, David A. ;
Trowsdale, John .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (15) :6200-6205
[8]   TRIM59, a novel multiple cancer biomarker for immunohistochemical detection of tumorigenesis [J].
Khatamianfar, Vida ;
Valiyeva, Fatma ;
Rennie, Paul S. ;
Lu, Wei-yang ;
Yang, Burton B. ;
Bauman, Glenn S. ;
Moussa, Madeleine ;
Xuan, Jim W. .
BMJ OPEN, 2012, 2 (05)
[9]   Wnt5a attenuates the pathogenic effects of the Wnt/β-catenin pathway in human retinal pigment epithelial cells via down-regulating β-catenin and Snail [J].
Kim, Joo-Hyun ;
Park, Seoyoung ;
Chung, Hyewon ;
Oh, Sangtaek .
BMB REPORTS, 2015, 48 (09) :525-530
[10]   Role of the focal adhesion protein TRIM15 in colon cancer development [J].
Lee, Ok-Hee ;
Lee, Jinkyoung ;
Lee, Keun Ho ;
Woo, Yun Mi ;
Kang, Ju-Hee ;
Yoon, Ho-Geun ;
Bae, Soo-Kyung ;
Songyang, Zhou ;
Oh, Seung Hyun ;
Choi, Youngsok .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2015, 1853 (02) :409-421
123